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RNA-SEQ NEWS

Featured RNA-Seq Job – NGS Scientist: Next Generation Diagnostics

21 hours ago 0 328 Views

Novartis Institutes for BioMedical Research About the Next Generation Sequencing Group The ability to sequence each patient’s genome in great detail, quickly and inexpensively, is changing the practice of medicine. ...

Next-Generation Sequencing RNA-Seq Library Construction

21 hours ago 0 262 Views

This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols ...

RNA-Seq Presentations – Available for Download

22 hours ago 0 227 Views

8 presentations from the RNA-Seq series so far. All nicely collated for you in a ‘Past Presentation Pack’. There are a number of presentations in there from our European RNA-Seq ...

Gene Expression, Single Nucleotide Variant and Fusion Transcript Discovery in Archival Material

2 days ago 0 509 Views

Advantages of RNA-Seq over array based platforms are quantitative gene expression and discovery of expressed single nucleotide variants (eSNVs) and fusion transcripts from a single platform, but the sensitivity for ...

Final Agenda Released – RNA-Seq 2014

March 7, 2014

Next Generation Sequencing Conference Highlights: Exomes, Genomes & Transcriptomes In Clinical Diagnostics

February 26, 2014

Molecular Med Tri-Con – Genome and Transcriptome Analysis

February 8, 2014

Advances in NGS & Big Data

February 6, 2014

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Jobs

Featured RNA-Seq Job – NGS Scientist: Next Generation Diagnostics

21 hours ago 0 328 Views

Novartis Institutes for BioMedical Research About the Next Generation Sequencing Group The ability to sequence each patient’s genome in great detail, quickly and inexpensively, is changing the practice of medicine. ...
Read More »
Featured RNA-Seq Job – Bioinformatics Data Analyst

6 days ago 0 1,036 Views
RNA-Seq Jobs – Linkedin

17 days ago 0 1,000 Views
Featured RNA-Seq Jobs

28 days ago 0 651 Views
RNA-Seq Postdoctoral Positons Available

February 20, 2014 0 602 Views

Industry News

Life Technologies Releases New Research Tool: Oncomine NGS RNA-Seq Gene Expression Browser

9 days ago 0 1,459 Views

SAN DIEGO–(BUSINESS WIRE)–Knowledge of driver mutations is essential to research and development in the pharmaceutical industry for the identification of novel populations and discovery of new cancer drug targets. Gene ...
Read More »
Illumina adds products to support RNA-Seq on the newly launched NextSeq™ 500 sequencing system

14 days ago 1 Comment 904 Views
Pacific Biosciences Releases Software Upgrade to Support Full-Length Transcript Sequencing

14 days ago 0 654 Views
Maverix Strengthens Strategic Co-Marketing Alliance With QIAGEN

15 days ago 0 555 Views
Pacific Biosciences Releases Software Upgrade to Support Full-Length Transcript Sequencing and HLA Haplotype Phasing

16 days ago 0 932 Views

SEQanswers - RNA Sequencing
Post Feed

High discordant alignments

I've set up a galaxy workflow for paired end first stranded RNAseq, and I've gotten some o [...]

Differential Expression across libraries/platforms

I've been searching for a while now and I can't seem to find any kind of discussion on thi [...]

could not find function "DESeqDataSetFromMatrix"

Hi I am trying to use DESeq2 for differential expression analysis. Everything goes fine: DESeq2 is i [...]

Reads mapped to another chromosome in paired-end data of RNA-seq

Hello guys, I am doing a project in RNA-seq data with some datasets that are paired-end data. After [...]

Correcting a reference genome for Tophat

Hi. I am currently mapping plant transcriptome reads from one cultivar to the genome of another culi [...]

HTseq-count of zero

Hi I ran htseq on a number of RNA expression data, but I get a read count of zero for a certain tran [...]

cummeRbund PCA plot

Can anyone explain cummeRbund's PCA plot? I do understand traditional PCA plots, like the one i [...]

Error Running DESeq2

Hi I am trying to run DESeq2 using the reference manual provided in the bioconductor website. Howeve [...]

GATK preprocessed bam files for cufflinks?

Hello all, I'm relatively new to rnaseq analysis. I've been reading a lot and currently tr [...]

How to identify antisense in lncRNA?

I have some human and mouse RNAseq and I going to identify lncRNA antisense for both of them. the da [...]

FPKM = RPKM for single end reads?

Hi Readers, I am a computer scientist and I am trying to understand the relationship between RPKM an [...]

any tools to test if my RNAseq data is enough?

Hi, I am new to the field of RNA sequencing.I have read a lot of message about how many reads is eno [...]

how to minimize transcripts while de novo assembly transcriptome

Hi all, I am using Trinity for de novo assembly of mRNA from Illumina Hiseq2000, 100bp pair ends. We [...]

SureSelect RNA versus TruSeq Targeted RNA

Hello all, Has anyone compared the gene expression quantification ability between SureSelect RNA and [...]

very low percent uniquely mapped for only one read out of the pair

Hello! I am baffled by this issue. I am having trouble getting any significant percentage of reads t [...]

Quantify ration of spliced to unspliced reads

Hello, I am interested in measuring the ratio of spliced to un-spliced genes in an RNA-seq experimen [...]

question about read depth for splice variant detection

Dear All, I plan to perform RNA-seq recently, but I am a newer to RNA-seq and I found it was hard to [...]

Unable to make heatmap in CummeRbund

Hi all, I tried to make a heatmap through CummeRbund, but was unable to complete it. This is what I [...]

Adapter trimming

Hi I have got the adapter sequences from company as for Illumina RNA seq data:... [...]

Separating reads from different organisms

My RNAseq data contains reads from two different organisms (a host and a pathogen). I wish to separa [...]

Transcriptome Wide Snps Based Sample Clustering

Dear all, I sequenced the trancriptome of 45 samples using Hiseq2000. Now, I intend to cluster these [...]

Cuffdiff Fpkm Values

Hello everyone, I am comparing two samples (control and treated) paired end RNA Seq data in Galaxy. [...]

Consistency Of Biological Samples In De Novo Transcriptome Assemblies

I've heard that it is generally good practice to assemble transcriptomes from libraries of simi [...]

News: Tuesday 10/29 Live Tour Of New Features In Integrated Genome Browser 8

Please join us for a live, on-line tutorial introducing IGB 8, a preview of an upcoming release of t [...]

Shrimp Uniquely Aligned Reads

Hello, I have used SHRiMP for aligned SOLiD RNA-Seq data using the following commands. $SHRIMP_FOLDE [...]

The Easiest/Fastest Way To Get From Bam To Tpm Or Rpkm

I have already aligned all my RNA-seq samples using tophat2. I've done the differential analysi [...]

Fast Rna-Seq Exon Count Calculation

Hi, I want to calculate exon counts from a large number of bam files. I have done this before using [...]

Determining Replicates For Deseq?

Hello, I am relatively new to RNA-Seq data analysis so I apologize in advance if this is a novice qu [...]

Validation Of De Novo Rna-Seq Assembly

Hi, I recently performed the de novo assembly of plant RNA-seq data using Velvet/Oases with the mult [...]

News: Survey: Rna-Seq Analysis For Differential Gene/Transcript Expression [Updated With Results]

Hi all, I am looking to build a 'standard' RNA-seq data analysis pipeline for analysing di [...]

De Novo Transcriptome And Comparative Genomics

Hello all, First time posting here. I am looking at doing some comparative genomics with a mammalian [...]

How To Get Equal Numbers Of Gene_Id And Exon_Id For Use In Newexoncountset Function For Dexseq

I'm trying to run DEXseq but am having trouble with the newExonCountSet function because my gen [...]

Deseq Standard Comparison Or Multifactorial / Model Fitting?

Hi, I'm analyzing a dataset with DESeq, which consists of two time-points and two genotypes. I [...]

Co-Expression Networks Of Rna-Seq

Hi everyone! I'm interested in making a co-expression network out of RNA-seq data that I have f [...]

Local Alignment Of Rna-Seq Data

Hi, Anyone knows a good tool to align RNA-Seq data in local alignment mode ? The tool has to be spli [...]

Tutorial: Rna Seq Experimental Design For Measuring Differential Gene Expression

I just want to call the attention to a remarkably detailed comment to a question that seems to be of [...]

Strategy For Rna Seq Normalization Across Different Tissue Samples With Different Cell Density.

Hello All, This is another question about RNA Seq data normalization. Often, I have read papers usin [...]

Mirna-Seq Datasets

Hi guys, I'm looking for some experiments of miRNA-seq in different tissues (essentially human [...]

When Using Deseq Does One Need To Estimate The Size Factor And Dispersion For Each Condition?

Hi all, I want to ask a question in using DESeq, do I also need to import two conditions each time t [...]

What Are Recommend Approaches For Studying Bacterial Rna-Seq Data

I have a data from Hi-seq for a bacterial RNA-seq , however, it is an over kill, with any aligner it [...]