Monthly Archives: September 2010

Upcoming Next-Gen Sequencing Conferences

Evolution of Next Generation Sequencing September 27th – 29th, 2010 Rhode Island Convention Center – Providence, RI Cambridge Healthtech Second-Generation Applications to Third-Generation Progress Beyond the Genome: The true gene count, human evolution and disease genomics October 11th – 13th, ...

Read More »

The Debate Rages On!

A systematic comparison of RNA-Seq and High-Density Exon Array for detecting differential gene expression between closely related species (a panel of human/chimpanzee/rhesus cerebellum RNA samples). Results indicate that RNA-Seq has significantly improved gene coverage and increased sensitivity for differentially expressed ...

Read More »

GOseq – Gene Ontology (GO) analysis on RNA-seq data

GO analysis is widely used to reduce complexity and highlight biological processes in genome-wide expression studies, but standard methods give biased results on RNA-seq data due to over-detection of differential expression for long and highly expressed transcripts. The authors have ...

Read More »

RNA-Seq Data Contributes to Major miRBase Update

The miRBase microRNA Sequence Database provides a searchable online repository for published microRNA sequences and associated annotation.  The database is updated 2 to 3 times per year based on publication of any new experimentally verified microRNA sequences.  The first version ...

Read More »

New Splice Junction Mapping Tool – MapSplice

The accurate mapping of reads that span splice junctions is a critical component of all analytic techniques that work with RNA-seq data. Here is a second generation splice detection algorithm, MapSplice, whose focus is high sensitivity and specificity in the ...

Read More »