Monthly Archives: August 2011

RNA sequencing biases caused by random hexamer priming

Researchers at UC Berkeley have shown that priming using random hexamers biases the nucleotide content of RNA-Seq reads and that this bias also affects the uniformity of the locations of the reads along expressed transcripts. Despite this bias, they suggest ...

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Allele-specific gene expression by RNA-seq

Variation in gene expression is thought to make a significant contribution to phenotypic diversity among individuals within populations. Although high-throughput cDNA sequencing offers a unique opportunity to delineate the genome-wide architecture of regulatory variation, new statistical methods need to be ...

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Ignored biases confound RNA-Seq results

High-throughput RNA sequencing (RNA-seq) continues to provide unparalleled insight into transcriptome complexity. Now the gold standard for assessing global transcript levels, RNA-seq is poised to revolutionize our understanding of transcription and post-transcriptional regulation of RNA. To fully capitalize on the ...

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