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Agilent Technologies Agilent Technologies

Agilent Technologies – Agilent’s SureSelect market leading platform provides a complete portfolio of catalog and custom products, providing the flexibility you need, for your discovery to follow-up studies. New HaloPlex products combine the speed of PCR with the sensitivity of hybridization providing a robust solution for targeting smaller capture regions. http://www.genomics.agilent.com/CollectionOverview.aspx?PageType=Application&SubPageType=ApplicationOverview&PageID=2094&CID=G0961   Incoming search terms:agilent [...]

Beckman Coulter Genomics Beckman Coulter Genomics

Beckman Coulter Genomics – Beckman Coulter Genomics RNA-Seq services ensure all your bases are covered providing an end-to-end solution including expert project design, automated Illumina* TruSeq* library construction, sequencing on the Illumina HiSeq* platforms and comprehensive data analysis. The new fully automated sample preparation pipeline delivers consistent results run after run reducing user variability and [...]

LC LC Sciences

LC Sciences – provides a one-stop solution for all your microRNA (miRNA) discovery and expression profiling needs.  High-throughput sequencing is now available in addition to existing microarray and qRT-PCR profiling services for the most complete picture of microRNA expression in your samples.  microRNA sequencing is a new method and a powerful tool to identify and [...]

Fluidigm Fluidigm

Fluidigm – The C1™ Single-Cell Auto Prep System is a new approach to single-cell genomic applications, including targeted gene expression and the new mRNA sequencing protocol for transcriptome analysis of single cells. http://www.fluidigm.com/c1system.html Incoming search terms:fluidigm dataset rna-seqlc sciences miRNA sequencingfluidigm publicationsillumina rna seq validation two peaksribosom profiling

Oxford Oxford Gene Technologies

Oxford Gene Technologies – A high-quality RNA sequencing service designed to deliver cost-effective and rapid access to meaningful transcriptomics results. http://www.ogt.co.uk/services/737_rna-seq_service  

Epicentre Epicentre (an Illumina company)

 - The Ribo-Zero™ Magnetic Gold Kit (Epidemiology) combines Epicentre’s Ribo-Zero™ Gold (Human/Mouse/Rat) and Ribo-Zero™ (Bacteria) rRNA removal reagents into a single solution that effectively removes rRNA, including human mitochondrial RNA, from samples composed of both human/mouse/rat and bacterial RNA. http://www.epibio.com/products/rna-sequencing/rrna-removal/ribo-zero-magnetic-gold-kit-%28epidemiology%29 Incoming search terms:epicentre illumina companyRibo-zerodna methylation epicentre illuminaillumina epicentre forumillumina epicentre low amount rnaseqkit real [...]

SevenBridges SevenBridges Genomics

SevenBridges Genomics – RNA-Seq with IGOR – A fully connected toolkit – Focus on your research. Get results faster. Build discovery pipelines from a large and fast growing arsenal of tools in our visual editor, or simply use IGOR’s library of peer-reviewed “gold standard” pipelines. To expand your toolkit even further, our SDK can integrate [...]

Lexogen Lexogen

Lexogen – The SENSE mRNA-Seq kit is an all-in-one library preparation protocol designed to generate Illumina-compatible libraries from total RNA within 4 hours. The SENSE protocol maintains strand-specificity and allows the mapping of reads to their corresponding strand on the genome, enabling the discovery and quantification of antisense transcripts and overlapping genes. http://www.lexogen.com/sense.html Incoming search [...]

NuGEN NuGEN Technologies

NuGEN Technologies – The Ovation Ultralow Methyl-Seq Library Systems provide a simple, fast and scalable solution for producing libraries used in conjunction with bisulfite sequencing to analyze DNA methylation. The system requires as little as 10 ng of DNA, thereby enabling methylation studies with a broad range of sample types. The protocol is compatible with [...]

Ingenuity Ingenuity Systems

Ingenuity Systems – Model, analyze, and understand the complex biological and chemical systems at the core of life science research with IPA. Peer Reviewed and Widely Adopted- IPA has been broadly adopted by the life science research community and is cited in thousands of peer-reviewed journal articles. Understand Complex ‘Omics Data – IPA helps you [...]

Perkin Perkin Elmer

PerkinElmer’s DNA Sequencing and Analysis Service provides scientists access to state-of-the-art next generation sequencing platforms with seamless integration to robust data analysis and visualization software from Geospiza. The seamless integration of sequencing, analysis and secure online data storage means you get “anytime, anywhere” access to your research, all backed by PerkinElmer’s industry expertise and the [...]

Strand Strand Scientific

Strand Scientific – Avadis NGS supports an extensive workflow for the analysis and visualization of RNA-Seq data. The workflow includes standard differential expression analysis for a variety of experiment conditions, as well as differential splicing analysis. It supports novel discovery steps including identifying novel genes and exons and novel splice junctions. It includes the ability [...]

Otogenetics Otogenetics

Otogenetics – Otogenetics offers complete RNA-Seq services at a price that is competitive to microarrary approaches.  We use RNA-Seq protocols that are extensively validated by Illumina’s R&D team, which is validated to have a dynamic range of at leat one million folds.  Our RNA-Seq services cover human and many other species. http://www.otogenetics.com/rnaseq_page_010812.htm Incoming search terms:otogeneticsotogenetics [...]

Expression Expression Analysis

Expression Analysis – mRNA-Seq is a cDNA sequencing application that generates a more comprehensive, quantitative view of the mRNA portion of the transcriptome. With no probes or primer design needed, RNA-Seq has the potential to provide relatively unbiased sequence information from polyA-tailed RNA for analysis of gene expression, novel transcripts, novel isoforms, alternative splice sites, [...]

Advanced Advanced Analytical

Advanced Analytical – The Fragment Analyzer™ Automated CE System provides an RQN measurement to assist with RNA analysis. RQN stands for RNA Quality Number. Derived from PROSize® analytical software, RQN assesses the quality or integrity of total RNA on a scale of 1-to-10, and allows for correlation to legacy data. http://www.aati-us.com/instruments/fragment_analyzer/applications/RNAanalysis/index.html Incoming search terms:advanced analytical [...]

Cofactor Cofactor Genomics

Cofactor Genomics – is a Contract Research Organization (CRO) that was formed in 2008 offering RNA sequencing services. http://www.cofactorgenomics.com/application/rna-seq Incoming search terms:map program cofactor genomics

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  • fluidigm oxford c1
  • fragment analyzer
  • sense lexogen
  • does otogenetics do strand specific rna seq
  • list of rna
  • ovation® ultralow methyl-seq library systems price
  • we will run correlations of the results from the rna-seq with results from the fluidigm to validate that our rna-seq expression results are real
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  • RSS SEQanswers – RNA Sequencing

    • RNAseq (SOLiD) from 18 - 200 nt June 18, 2013
      We are interested in small non-coding RNAs. Whomever you ask about the size range of small RNAs, you get a different answer. ;) Lets assume, small... […]
      GenomicIBK
    • Unmapped ratio very high on mouse genome June 17, 2013
      Hi, My problem regards RNA-Seq data. I've downloaded public data (SAGE libs w/ 6 different samples from mouse liver ) to analyse using ArrayStudio.... […]
      le.nono
    • RNASeq: Read length different from expected June 17, 2013
      Hello all, I have received paired-end reads for 40 samples. The reads are supposed to be 100bp per end. Instead, 20 of my samples are 101bp per... […]
      gogodidi
    • How to install xgawk June 16, 2013
      Hi, This is Shrujan, i have a problem while running RNA Sequencing QC. It shows an error that xgawk is not found. So please help me installing... […]
      shrujan
    • RNA Sequencing QC Error while using with Sequence_QC.sh file June 15, 2013
      Hi, This is Shrujan kumar Madadha, I had an error while running QC for Drosophila Yukuba fastq RNA file using Sequence_QC.sh file of FASTX... […]
      shrujan
    • Cuffmerge related query June 12, 2013
      I have a query regarding what samples should be merged using cuffmerge, when you have multiple phenotypes (each with replicates). Lets say my mouse... […]
      ParthavJailwala
  • RSS Biostar – RNA-Seq

    • edgeR: very low p-value and very high variance within the group of replicates. What's my problem??
      I'm using edgeR in order to perform differential expression analysis from RNA-seq experiment. I have 6 samples of tumor cell, same tumor and same treatment: 3 patient with good prognosis and 3 patient with bad prognosis. I want to compare the gene expression among the two groups. I ran the edgeR pakage like follow: x […]
    • Normalising tag count to RPKM
      Hi! I was wondering if their is a way to normalise the number of reads in a region and the RPKM of the nearest gene to that region, so that a correlation could be computed. Like the following data shows number of tags in first column and RPKM in second column Tags RPKM 15 0.14619 11 0 203 0.2259 129 10.701 300 7.0772 122 2.3234 346 10.666 77 3.117 201 16.749 […]
    • a simple question on RNA-Seq terminology
      This question may be very simple and basic, but I just need to confirm that I understand the differences among those terminologies in the RNA-Seq context. Suppose I have a sample called SLR, and it is sequenced on 5 lanes, so I have (among other output files) BAM files like L1_SLR, L2_SLR, L3_SLR, L5_SLR and L7_SLR.bam. Here, the letter "L" denotes […]
    • FInding regions of interest with minimum coverage
      Hi, I have a bam file of all my accepted hits (tophat output) and an gtf file with my genes of interest for which I am trying to find potential antisense transcripts. I would like to create a list - preferably one that can be visualized in a genome browser - that shows all genes that have antisense reads in the accepted hits.bam file provided that there are […]
    • How to remove the intronic reads before counting
      I got RNASeq data in several samples. I checked the FastQC, seems the read quality are good (Hiseq 2000). But the problem is many reads are mapped to intronic region, and the regions have no any reference exons there (Refseq, ensembl, gencode). We don't know what they are. We guess the problem happend in library preparation, the concentration was low. N […]
    • Which strand of the mRNA molecule does the sequencer output as a "read"?
      In Illumina Stranded RNA-Seq (using the dUTP method), do the final reads in the fastq files correspond to the initial molecule (that was transcribed), or to the reverse complement of the molecule? C […]