AG-NGS: semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms

Next-generation sequencing (NGS) is becoming one of the most widely used technologies in the field of genomics. Library preparation is one of the most critical, hands-on, and time-consuming steps in the NGS workflow. Each library must be prepared in an independent well, increasing the number of hours required for a sequencing run and the risk of human-introduced error. Automation of library preparation is the best option to avoid these problems.

With this in mind, researchers from the Spanish National Center for Cardiovascular Research (CNIC), Spain have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experience, and the application has also been designed to minimize implementation costs. Automated library preparation with AG-NGS generated high-quality libraries from different samples, demonstrating its efficiency, and all quality control parameters fell within the range of optimal values.

rna-seq

Callejas S, Alvarez R, Benguria A, Dopazo A. (2014) AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms. Biotechniques 56(1), 28-35. [article]