Approaches to querying bacterial genomes with transposon-insertion sequencing

In this review, the authprotocols that have been developed and methods for analysis and provide an overview of studies that have examined the reproducibility and accuracy of these methods, as well as studies showing the advantages offered by the high resolution and dynamic range of high-throughput sequencing over previous methods. The authors go on to review a number of applications in the literature, from predicting genes essential for in vitro growth to directly assaying requirements for survival under infective conditions in vivo. They also highlight recent progress in assaying non-coding regions of the genome in addition to known coding sequences, including the combining of RNA-Seq with high-throughput transposon mutagenesis.

transposon-insertion sequencing

  • Barquist L, Boinett CJ, Cain AK. (2013) Approaches to querying bacterial genomes with transposon-insertion sequencing. RNA Biol 10(7). [abstract]