Data Analysis

Accurate mapping of tRNA reads

rna-seq

Many repetitive DNA elements are transcribed at appreciable expression levels. Mapping the corresponding RNA sequencing reads back to a reference genome is notoriously difficult and error-prone task, however. This is in particular true if chemical modifications introduce systematic mismatches, while ...

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Eliminating platform-based bias

rna-seq

Molecular subtypes of cancers and autoimmune disease, defined by transcriptomic profiling, have provided insight into disease pathogenesis, molecular heterogeneity, and therapeutic responses. However, technical biases inherent to different gene...

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DEWE – Differential Expression Workflow Executor for RNA-Seq

rna-seq

DEWE (http://www.sing-group.org/dewe/) is an open source application for easily executing Differential Expression analyses in RNA-Seq data. DEWE offers built-in, easy-to-configure workflows that facilitate the execution of DE analyses. Currently, DEWE provides two differential expression analysis workflows: HISAT2, StringTie and Ballgown ...

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