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FRAMA – from RNA-seq data to annotated mRNA assemblies

FRAMA – from RNA-seq data to annotated mRNA assemblies

Advances in second-generation sequencing of RNA made a near-complete characterization of transcriptomes affordable. However, the reconstruction of full-length mRNAs via de novo RNA-seq assembly is still difficult due to the complexity of eukaryote transcriptomes with highly similar paralogs and multiple ...

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SATRAP – SOLiD Assembler TRAnslation Program

SATRAP – SOLiD Assembler TRAnslation Program

SOLiD DNA sequences are typically analyzed using a reference genome, while they are not recommended for de novo assembly of genomes or transcriptomes. This is mainly due to the difficulty in translating the SOLiD color-space data into normal base-space sequences. ...

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RNA-Seq Analysis with STAR

RNA-Seq Analysis with STAR

NASB2015 Lecture 21- RNA seq Analysis with STAR In this lecture Yan Kou from the Ma’ayan Lab demonstrates how to run the popular genome aligner STAR for RNA-seq analysis

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