Transcriptome Assembly Tools

Feed Subscription

FANSe2: A Robust Tool for Quantitative NGS Applications

FANSe2: A Robust Tool for Quantitative NGS Applications

Correct and bias-free interpretation of the deep sequencing data is inevitably dependent on the complete mapping of all mappable reads to the reference sequence, especially for quantitative RNA-seq applications. Seed-based algorithms are generally slow but robust, while Burrows-Wheeler Transform (BWT) ...

Read More »

Hobbes2 – for fast and accurate alignment of NGS reads

Hobbes2 – for fast and accurate alignment of NGS reads

Next-generation sequencing (NGS) enables rapid production of billions of bases at a relatively low cost. Mapping reads from next-generation sequencers to a given reference genome is an important first step in many sequencing applications. Popular read mappers, such as Bowtie ...

Read More »

SHEAR: sample heterogeneity estimation and assembly by reference

SHEAR: sample heterogeneity estimation and assembly by reference

Personal genome assembly is a critical process when studying tumor genomes and other highly divergent sequences. The accuracy of downstream analyses, such as RNA-seq and ChIP-seq, can be greatly enhanced by using personal genomic sequences rather than standard references. Unfortunately, ...

Read More »

Sequencing error correction without a reference genome

Sequencing error correction without a reference genome

Next (second) generation sequencing is an increasingly important tool for many areas of molecular biology, however, care must be taken when interpreting its output. Even a low error rate can cause a large number of errors due to the high ...

Read More »
Scroll To Top