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To date there has been no curated list of NGS methods excepting Illumina’s “For all you seq” posters. Researchers at AstraZeneca have created an open-access Wiki (http://www.enseqlopedia.com/enseqlopedia) that describes over 300 methods and lists relevant publications, including the primary reference.

CMU Software Assembles RNA Transcripts More Accurately 1,584 Views Researchers produce the first draft cell atlas of the small intestine 1,621 Views ExpressionDB – An open source platform for distributing genome-scale datasets 1,768 Views RefEx – a reference gene expression ...

You may have heard about the wonderful things you can find in the raw data your lab produces. Some of it gets published: it becomes part of a groundbreaking study, or inspires the development of a life-saving drug, though most ...

Despite RNA-Seq being the capstone technology for gene expression profiling, qPCR remains the go-to technique for validation. To this day, qPCR validation of microarray data is commonplace, and many see it as necessary, given that microarrays involve hybridization to a glass ...

RNA sequencing (RNA-Seq) and microarray are two of the most commonly used high-throughput technologies for transcriptome profiling; however, they both have their own inherent strengths and limitations. Researchers from Fudan University aimed to analyze the correlation between microarrays and RNA-Seq detection of ...

News Medical Life Sciences by By Catherine Shaffer, M.Sc. – If DNA is the fundamental code of the genome, RNA is the message. Genes are made active through the process of transcribing the code into RNA, and then translating RNA into DNA. This is known as ...

RNA sequencing (RNA-seq) and microarrays are two transcriptomics techniques aimed at the quantification of transcribed genes and their isoforms. Here researchers from the Luxembourg Institute of Health compare the latest Affymetrix HTA 2.0 microarray with Illumina 2000 RNA-seq for the ...

We’ve been keeping track of the number of papers that we can find citing RNA-Seq since 2008.  January is often a time to reflect back on the previous year, so how did the number of publications in 2016 compare to ...

Post Title Categories Views IntSplice – prediction of the splicing consequences of intronic single-nucleotide variations in the human genome Splicing and Junction Mapping 7,236 Comparative analysis of single-cell RNA-sequencing methods Publications, Reader Conributions 6,479 How many biological replicates are needed ...