Sequencing Protocols

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DrugTargetSeqR: a genomics- and CRISPR-Cas9-based method to analyze drug targets

DrugTargetSeqR: a genomics- and CRISPR-Cas9-based method to analyze drug targets

To identify physiological targets of drugs and bioactive small molecules, researchers at The Rockefeller University developed an approach, named DrugTargetSeqR, which combines high-throughput sequencing, computational mutation discovery and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9-based genome editing. They applied this ...

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Step by Step – RNA-Seq

Step by Step – RNA-Seq

The introduction of RNA sequencing (RNA-seq), as a direct result of rapid progression of next-generation sequencing technologies, has revolutionized the world of transcriptomics. It enables quantification of the complete set of RNA with all its isoforms in a given cell, ...

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RNA sequencing in situ

RNA sequencing in situ

Writing in Science, Lee et al. report an exciting first step toward combining some of the best aspects of both methods to sequence RNA in single cells in situ. Their approach, called fluorescence in situ RNA sequencing (FISSEQ), effectively treats ...

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High-resolution transcriptome analysis with long-read RNA sequencing

High-resolution transcriptome analysis with long-read RNA sequencing

RNA sequencing (RNA-seq) enables characterization and quantification of individual transcriptomes as well as detection of patterns of allelic expression and alternative splicing. Current RNA-seq protocols depend on high-throughput short-read sequencing of cDNA. However, as ongoing advances are rapidly yielding increasing ...

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Simultaneous Transcriptional Profiling of Bacteria and Their Host Cells

Simultaneous Transcriptional Profiling of Bacteria and Their Host Cells

Researchers at the University of Maryland School of Medicine have developed an RNA-Seq-based method to simultaneously capture prokaryotic and eukaryotic expression profiles of cells infected with intracellular bacteria. As proof of principle, this method was applied to Chlamydia trachomatis-infected epithelial ...

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