Sequencing Protocols

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Simultaneous Transcriptional Profiling of Bacteria and Their Host Cells

Simultaneous Transcriptional Profiling of Bacteria and Their Host Cells

Researchers at the University of Maryland School of Medicine have developed an RNA-Seq-based method to simultaneously capture prokaryotic and eukaryotic expression profiles of cells infected with intracellular bacteria. As proof of principle, this method was applied to Chlamydia trachomatis-infected epithelial ...

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Significant differences in expression between individual cells

Significant differences in expression between individual cells

Single-cell RNA-seq mammalian transcriptome studies are at an early stage in uncovering cell-to-cell variation in gene expression, transcript processing and editing, and regulatory module activity. Despite great progress recently, substantial challenges remain, including discriminating biological variation from technical noise. Here ...

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RNA sequencing by primer extension

RNA sequencing by primer extension

Direct sequencing of RNA by primer extension is a fast and accurate method that is useful in a variety of situations. It is a valuable technique for determining the faithfulness of in vitro processing reactions, such as splicing or RNA ...

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Bacterial RNA sequencing workflow for PGM™

Bacterial RNA sequencing workflow for PGM™

Over the years, we at Ambion receive a number of requests for information about the best way to isolate and ribo-deplete prokaryotic total RNA for next-gen sequencing.  I just wanted to give a brief overview and some suggestions on RNA ...

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Accuracy of RNA-Seq and its dependence on sequencing depth

Accuracy of RNA-Seq and its dependence on sequencing depth

The cost of DNA sequencing has undergone a dramatical reduction in the past decade. As a result, sequencing technologies have been increasingly applied to genomic research. RNA-Seq is becoming a common technique for surveying gene expression based on DNA sequencing. ...

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