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NGS pipelines in UGENE

NGS pipelines in UGENE

UGENE is a free open-source cross-platform bioinformatics software. It works perfectly on Windows, Mac OS and Linux and requires only a few clicks to install. This video shows a brief overview of NGS pipelines in UGENE: “Variant Calling with SAMtools”, ...

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RNA-Seq Presentations – Available for Download

RNA-Seq Presentations – Available for Download

8 presentations from the RNA-Seq series so far. All nicely collated for you in a ‘Past Presentation Pack’. There are a number of presentations in there from our European RNA-Seq meeting (which took place in December last year), giving you ...

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Inferring UTR length using RNA sequencing data

Inferring UTR length using RNA sequencing data

This tutorial describes how to infer UTR length from RNA sequencing data. Use the GBrowse ruler function to measure the difference between a gene’s annotated start and the start of RNASeq reads. The difference estimates UTR length.

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RNA-Seq with EPIC-CoGe in 3 minutes

RNA-Seq with EPIC-CoGe in 3 minutes

This video tutorial shows how to clean and map RNA-Seq data to any genome in CoGe. This includes generating BAM files, quantitating reads per position across a genome, and generating FPKM for genes. These data can be kept private and ...

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RNA-Seq Shows its Sensitive Side

RNA-Seq Shows its Sensitive Side

Next generation sequencing has led to diverse RNA-Seq methods, but one drawback is sensitivity. Conventional RNA-Seq requires more than 10 ng of RNA from 50–100 cells. But increasingly researchers are interested in profiling gene expression in single cells. Ramskold and ...

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