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High-Resolution Transcriptome Analysis with Long-Read RNA Sequencing

High-Resolution Transcriptome Analysis with Long-Read RNA Sequencing

RNA sequencing (RNA-seq) enables characterization and quantification of individual transcriptomes as well as detection of patterns of allelic expression and alternative splicing. Current RNA-seq protocols depend on high-throughput short-read sequencing of cDNA. However, as ongoing advances are rapidly yielding increasing ...

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The evolution of black widow venom

The evolution of black widow venom

Black widow venom contains α-latrotoxin, infamous for causing intense pain. Combining 33kb of Latrodectus hesperus genomic DNA with RNA-Seq, we characterized the α-latrotoxin gene and discovered a paralog, 4.5kb downstream. Both paralogs exhibit venom gland specific transcription, and may be ...

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RNA Sequencing Analysis Reveals HeLa Cell Contamination

RNA Sequencing Analysis Reveals HeLa Cell Contamination

Nasopharyngeal carcinoma (NPC) cell lines are important model systems for analyzing the complex life cycle and pathogenesis of Epstein-Barr virus (EBV). Using an RNA-seq-based approach, researchers from Tulane University Health Sciences Center found HeLa cell contamination in several NPC cell ...

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International Review of Neurobiology – The Brain Transcriptome

International Review of Neurobiology – The Brain Transcriptome

The Brain Transcriptome International Review of Neurobiology – Volume 116, Pages 2-352 (2014) Chapter One – Introduction to Sequencing the Brain Transcriptome Pages 1-19 Robert Hitzemann, Priscila Darakjian, Nikki Walter, Ovidiu Dan Iancu, Robert Searles, Shannon McWeeney Chapter Two – ...

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Transcriptome Sequencing reveals that species diversity is predictable

Transcriptome Sequencing reveals that species diversity is predictable

Genetic diversity is the amount of variation observed between DNA sequences from distinct individuals of a given species. This pivotal concept of population genetics has implications for species health, domestication, management and conservation. Levels of genetic diversity seem to vary ...

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