Unspliced Mapping Tools

Comprehensive benchmarking of RNA-Seq aligners

Alignment is the first step in most RNA-seq analysis pipelines, and the accuracy of downstream analyses depends heavily on it. Unlike most steps in the pipeline, alignment is particularly amenable to benchmarking with simulated data. Researchers from the University of ...

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Optimizing RNA-Seq Mapping with STAR

rna-seq

Sequencing of transcribed RNA molecules (RNA-seq) is an invaluable tool for studying cell transcriptomes at high resolution and depth. RNA-seq datasets typically consist of tens to hundreds of millions of relatively short (30–200 nt) sequence fragments of the original RNA ...

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Fine-mapping cellular QTLs with RASQUAL and ATAC-seq

When cellular traits are measured using high-throughput DNA sequencing, quantitative trait loci (QTLs) manifest as fragment count differences between individuals and allelic differences within individuals. Researchers from the Wellcome Trust Sanger Institute have developed RASQUAL (Robust Allele-Specific Quantitation and Quality Control), ...

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