Technology

Poly-A selection or Ribo-depletion?

rna-seq

RNA sequencing (RNA-seq) has become an indispensable tool to identify disease associated transcriptional profiles and determine the molecular underpinnings of diseases. However, the broad adaptation of the methodology into the clinic is still hampered by inconsistent results from different RNA-seq ...

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RNA-Seq Identifies Circular RNAs in Arabidopsis

Leaf growth and senescence are controlled by tight genetic factors involved regulation at multiple levels. Circular RNAs (circRNAs) have recently been reported as the microRNA sponge to accomplish corresponding regulatory roles. This study aims to explore the expression profile and ...

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Predicting Gene Function and Protein-Protein Interactions with RNA-Seq Co-expression Data

rna-seq

This presentation is by Hyojin Lee, an undergraduate student from Princeton University. Hyojin describes her summer research project with the BD2K-LINCS DCIC in the Ma’ayan Lab at the Icahn School of Medicine at Mount Sinai. http://lincs-dcic.org/summer-research… http://icahn.mssm.edu/labs/maayan http://amp.pharm.mssm.edu/archs4/ https://github.com/MaayanLab/predicti… Abstract ...

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An evaluation of high-throughput isomiR identification tools

MicroRNAs carry out post-transcriptional gene regulation in animals by binding to the 3′ untranslated regions of mRNAs, causing their degradation or translational repression. MicroRNAs influence many biological functions, and dysregulation can therefore disrupt development or even cause death. High-throughput sequencing ...

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