Technology

RNA-Seq Study Design calculator

The purpose of this calculator is to assist with RNA-Seq study design, in particular with the determination of the number of samples required to ensure sufficient statistical power. In general, a paired design will ensure greater power, but at increased ...

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Protocol – Computational Analysis of RNA-Seq

Accurately and comprehensively mapping millions of relatively short reads to a reference genome sequence can require not only specialized software, but also more structured and automated procedures to manage, analyze, and visualize the data. Additionally, the computational hardware required to ...

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A new strategy for mapping RNA-Seq reads

Accurate estimation of expression levels from RNA-Seq data entails precise mapping of the sequence reads to a reference genome. Because the standard reference genome contains only one allele at any given locus, reads overlapping polymorphic loci that carry a non-reference ...

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Introducing Bulked Segregant RNA-Seq (BSR-Seq)

Bulked segregant analysis (BSA) is an efficient method to map genes responsible for mutant phenotypes. BSR-Seq makes use of RNA-Seq reads to efficiently map genes even in populations for which no polymorphic markers have been previously identified. BSR-Seq provides not ...

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