Technology

RNA-Seq and Rattlesnakes

Animal toxins represent a valuable source of pharmacologically active macromolecules, a unique system for studying molecular adaptation, and a powerful framework for examining structure-function relationships in proteins. Snake venoms in particular represent a tremendous opportunity to study a large, integrated ...

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NEUMA – estimating mRNA abundances from RNA-Seq data

NEUMA (Normalization by Expected Uniquely Mappable Area) is a novel, efficient and intuitive approach of estimating mRNA abundances from the whole transcriptome shotgun sequencing (RNA-Seq) data. NEUMA, is based on effective length normalization using uniquely mappable areas of gene and ...

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Nice Review of Advances in RNA-Seq Data Analysis

from Nature Biotechnology Two methods for de novo transcriptome assembly of short reads were published this year from Lior Pachter and colleagues1 and from Aviv Regev and colleagues2. The transcriptome can be analyzed by sequencing cDNA reverse transcribed from RNA ...

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RNA sequencing: advances, challenges and opportunities

In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA ...

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