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A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas

A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas

Although the function of the mammalian pancreas hinges on complex interactions of distinct cell types, gene expression profiles have primarily been described with bulk mixtures. Here researchers from the Israel Institute of Technology implemented a droplet-based, single-cell RNA-seq method to ...

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Sequencing Mitochondrial Transcriptomes with RNA-Seq

Sequencing Mitochondrial Transcriptomes with RNA-Seq

Thousands of mitochondrial genomes have been sequenced, but there are comparatively few available mitochondrial transcriptomes. This might soon be changing. High-throughput RNA sequencing (RNA-Seq) techniques have made it fast and cheap to generate massive amounts of mitochondrial transcriptomic data. Here, ...

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RNA sequencing of the nephron transcriptome: a technical note

RNA sequencing of the nephron transcriptome: a technical note

To understand the functions of the kidney, the transcriptome of each part of the nephron needs to be profiled using a highly sensitive and unbiased tool. RNA sequencing (RNA-seq) has revolutionized transcriptomic research, enabling researchers to define transcription activity and ...

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