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Sequencing Mitochondrial Transcriptomes with RNA-Seq

Sequencing Mitochondrial Transcriptomes with RNA-Seq

Thousands of mitochondrial genomes have been sequenced, but there are comparatively few available mitochondrial transcriptomes. This might soon be changing. High-throughput RNA sequencing (RNA-Seq) techniques have made it fast and cheap to generate massive amounts of mitochondrial transcriptomic data. Here, ...

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RNA sequencing of the nephron transcriptome: a technical note

RNA sequencing of the nephron transcriptome: a technical note

To understand the functions of the kidney, the transcriptome of each part of the nephron needs to be profiled using a highly sensitive and unbiased tool. RNA sequencing (RNA-seq) has revolutionized transcriptomic research, enabling researchers to define transcription activity and ...

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RNA-Seq Improves the Giant Panda Genome

RNA-Seq Improves the Giant Panda Genome

High-quality and complete gene models are the basis of whole genome analyses. The giant panda (Ailuropoda melanoleuca) genome was the first genome sequenced on the basis of solely short reads, but the genome annotation had lacked the support of transcriptomic ...

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Getting Better at RNA-Seq

Getting Better at RNA-Seq

RNA-seq enables gene expression profiling in selected spatiotemporal windows and yields massive sequence information with relatively low cost and time investment, even for non-model species. However, there remains a large room for optimizing its workflow, in order to take full ...

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