Small non-coding RNAs (ncRNAs) such as microRNAs, snoRNAs and tRNAs are a diverse collection of molecules with several important biological functions. Current methods for high-throughput sequencing for the first time offer the opportunity to investigate the entire ncRNAome in an essentially unbiased way. However, there is a substantial need for methods that allow a convenient analysis of these overwhelmingly large data sets.
Exploring small RNA biology or characterizing differential expression profiles by sequencing and comparing small RNA transcriptomes is also an exciting possibility to get more and deeper information about the world of non-coding RNAs (ncRNAs).
DARIO is a free web service that allows to study short read data from small RNA-seq experiments. It provides a wide range of analysis features, including quality control, read normalization, ncRNA quantification and prediction of putative ncRNA candidates.
The DARIO web site can be accessed at http://dario.bioinf.uni-leipzig.de/.
Fasold M, Langenberger D, Binder H, Stadler PF, Hoffmann S. (2011) DARIO: a ncRNA detection and analysis tool for next-generation sequencing experiments. Nucleic Acids Res [Epub ahead of print]. [article]