Dolomite Bio’s Single Cell RNA-Seq System Enables High Speed Encapsulation

Dolomite Bio has developed the Single Cell RNA-Seq System for efficient, high throughput single cell transcriptomics. This compact, scalable system enables rapid, reproducible droplet encapsulation of individual cells, allowing up to 10,000 single cell libraries to be generated in one 15-minute run.

rna-seqThe Single Cell RNA-Seq System allows up to 500 µl samples of cells and oligo-barcoded beads to be encapsulated in extremely monodisperse droplets, each containing a single cell. It incorporates all the components required to encapsulate individual cells, including the hardware – pumps, flow sensors, connectors and a high speed camera and microscope – advanced Flow Control Center software and accessories. The system uses a glass Single Cell RNA-Seq Chip – a commercially available version of the prototype PDMS Drop¬seq chip described by Macosko et al1 – offering faster run time, longer life and greater ease of use. Installation and user training is included with each system, ensuring straightforward implementation and rapid initiation of droplet encapsulation experiments. Following encapsulation, the cell is lysed, allowing the mRNA to be captured on the beads for downstream recovery and sequencing.

About Dolomite Bio
Dolomite Bio creates innovative products for high throughput single cell research. By encapsulating single cells in microfluidic droplets, our products enable rapid analysis of thousands or millions of individual cells and their biological products.

Dolomite Bio is a brand of Blacktrace Holdings Ltd (www.blacktrace.com), a world leader in Productizing Science®. Our Head Office is based in Royston (near Cambridge), UK and we have regional offices in the USA, Japan, India and Brazil.

Dolomite Bio’s products include µEncapsulator System and RNA Seq system, suitable for use in many applications such as isolating antibody coding sequencing, profiling T cell receptors, droplets for FACS-sorting libraries and encapsulating cells in gel microbeads. To find out more about Dolomite Bio, please visit www.dolomite-bio.com

Macosko EZ, Basu A, Satija R, Nemesh J, Shekhar K, Goldman M, Tirosh I, Bialas AR, Kamitaki N, Martersteck EM, Trombetta JJ, Weitz DA, Sanes JR, Shalek AK, Regev A, McCarroll SA. (2015) Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell 161(5):1202-14. [article]

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