Clinical Genomics

RNA-Seq and Transcriptome Analysis Conference

Posted on May 6th, 2013

Cambridge Healthtech Institute and Bio-IT World’s Inaugural RNA-Seq and Transcriptome Analysis Part of the Fifth International Clinical Genomics…

Continue Reading

university-of-illinois

Workshop on RNA-Seq data analysis

Posted on May 6th, 2013

From Raw Sequence Data To mining Functional Information From Gene Lists Using Galaxy And R The workshop is currently full. However, if there is…

Continue Reading

QFAB

RNA-Seq analysis using Galaxy

Posted on May 3rd, 2013

Event:         RNA-Seq analysis using GalaxyStart:          July 4, 2013 9:00 amEnd:            July 4, 2013 5:30…

Continue Reading

GTEx Project

Genotype-Tissue Expression Project Community Meeting

Posted on May 2nd, 2013

The goal of the first GTEx Project Community Meeting is to share information about the project and to showcase advances made during the project's…

Continue Reading

nbic

Introductory Course – The Power of RNA-seq

Posted on May 1st, 2013

General info Date - 05 Jun 2013 - 07 Jun 2013 Location - Wageningen, the NetherlandsKeywords - RNA-seq, transcriptome, experimental design,…

Continue Reading

uc-riverside

Seminar – RNA-Seq for entomologists: addressing questions of sequencing depth and tissue specificity

Posted on May 1st, 2013

Dr.Brian Johnson, Department of Entomology & Nematology, UC Davis Monday, May 6, 2013 4:10–5 p.m. Location: Genomics Building 1102A Parking…

Continue Reading

aacr

Back from AACR

Posted on Apr 12th, 2013

Just returned from a great week at the American Association for Cancer Research (AACR) annual meeting and RNA-Seq was a big topic there.  Saw some…

Continue Reading

Your comprehensive guide to applying RNA-Seq in research and drug discovery

Posted on Apr 9th, 2013

Attending RNA-Seq 2013 will arm you with the knowledge and skills you need to optimize the practical application of RNA-Seq technology in your…

Continue Reading

rna-seqsummit

Early Registration Closing Friday – RNA-Seq 2013

Posted on Mar 26th, 2013

You have until this Friday 29th March to register for RNA-Seq 2013 (18-20 June, Boston) at the best possible rates. RNA-Seq 2013 brings together…

Continue Reading

UCDavis

April 11, 2013 Boot Camp – Introduction to RNAseq

Posted on Mar 6th, 2013

Bioinformatics courses, boot camps and workshops presented by the University of California, Davis Bioinformatics Core RNA-seq has emerged as a…

Continue Reading

XGEN_200x100

3rd Annual RNA-Sequencing Conference – Special Offer to Blog Readers

Posted on Mar 5th, 2013

The RNA-Seq Blog has formed a partnership with CHI, and as a special offer to readers of the blog, CHI will offer you a partner discount – a…

Continue Reading

cri

Workshop – Analyzing Illumina RNA-­seq Data with the CRI

Posted on Feb 25th, 2013

Title - Analyzing Illumina RNA-­seq Data with the CRI Instructor - Elizabeth Bartom, PhD and Lei Huang, PhD Location - Biological Sciences…

Continue Reading

5575-rna-seq-logo

Final agenda announced – RNA-Seq 2013

Posted on Feb 21st, 2013

Your comprehensive guide to applying RNA-Seq in research and drug discovery Attending RNA-Seq 2013 will arm you with the knowledge and skills you…

Continue Reading

rna-seq-crop

RNA-Seq Conference 2013 – Advanced approaches for gathering, interpreting & applying next generation transcriptome data

Posted on Feb 1st, 2013

Unlimited discovery of the entire RNA universe. Unbiased and unparalleled insights into the active genome. Whether it’s a need for more accurate…

Continue Reading

qpcr

qPCR & NGS 2013 – Early Bird Registration Peroid & Abstract Call

Posted on Jan 28th, 2013

6th international qPCR & Next Generation Sequencing Event Symposium  &  Industrial Exhibition  &  Application Workshops 18th - 22…

Continue Reading

bioinformatics

RNA-seq Bioinformatics – A Practical Introduction

Posted on Jan 24th, 2013

Mapping, Visualization, Basic Analyses May 13th - 14th 2013, Leipzig, Germany Scope and Topics The purpose of this workshop is to get a…

Continue Reading

research-computing

Short Training Class – RNA-seq Analysis in Galaxy – Slide Handouts

Posted on Jan 23rd, 2013

For those who missed it... here are the slides from the recent short training class presented by the Bioinformatics & Research Computing group at…

Continue Reading

Untitled-4

RNA-Seq – a big topic at next week’s Plant & Animal Genomes Conference

Posted on Jan 7th, 2013

Identification of the Chromosomal Region Controlling Naringenin Chalcone Accumulation in Melon Rind by a Combination of RNA-Seq and Bulk Segregant…

Continue Reading

b&g

RNA-Seq Workshop – An introductory course to RNA-Seq

Posted on Jan 4th, 2013

Centro di Biotecnologie Molecolari, Via Nizza 52, Torino, Italy, 27-28 March 2013 Next Generation Sequencing platforms changes the conventional…

Continue Reading

x-gen

REMINDER – 3rd Annual RNA-Seq Conference Registration Deadline is Friday, Dec 14

Posted on Dec 17th, 2012

To view the agenda and registration options, visit http://www.xgencongress.com/RNA-Seq 3rd Annual RNA-Seq: Differential Expression in Depth…

Continue Reading

  • Social Networking Pages

    Linkedin Group

  • Follow Me on Pinterest
  • RSS SEQanswers – RNA Sequencing

    • RNAseq (SOLiD) from 18 - 200 nt June 18, 2013
      We are interested in small non-coding RNAs. Whomever you ask about the size range of small RNAs, you get a different answer. ;) Lets assume, small... […]
      GenomicIBK
    • Unmapped ratio very high on mouse genome June 17, 2013
      Hi, My problem regards RNA-Seq data. I've downloaded public data (SAGE libs w/ 6 different samples from mouse liver ) to analyse using ArrayStudio.... […]
      le.nono
    • RNASeq: Read length different from expected June 17, 2013
      Hello all, I have received paired-end reads for 40 samples. The reads are supposed to be 100bp per end. Instead, 20 of my samples are 101bp per... […]
      gogodidi
    • How to install xgawk June 16, 2013
      Hi, This is Shrujan, i have a problem while running RNA Sequencing QC. It shows an error that xgawk is not found. So please help me installing... […]
      shrujan
    • RNA Sequencing QC Error while using with Sequence_QC.sh file June 15, 2013
      Hi, This is Shrujan kumar Madadha, I had an error while running QC for Drosophila Yukuba fastq RNA file using Sequence_QC.sh file of FASTX... […]
      shrujan
    • Cuffmerge related query June 12, 2013
      I have a query regarding what samples should be merged using cuffmerge, when you have multiple phenotypes (each with replicates). Lets say my mouse... […]
      ParthavJailwala
  • RSS Biostar – RNA-Seq

    • edgeR: very low p-value and very high variance within the group of replicates. What's my problem??
      I'm using edgeR in order to perform differential expression analysis from RNA-seq experiment. I have 6 samples of tumor cell, same tumor and same treatment: 3 patient with good prognosis and 3 patient with bad prognosis. I want to compare the gene expression among the two groups. I ran the edgeR pakage like follow: x […]
    • Normalising tag count to RPKM
      Hi! I was wondering if their is a way to normalise the number of reads in a region and the RPKM of the nearest gene to that region, so that a correlation could be computed. Like the following data shows number of tags in first column and RPKM in second column Tags RPKM 15 0.14619 11 0 203 0.2259 129 10.701 300 7.0772 122 2.3234 346 10.666 77 3.117 201 16.749 […]
    • a simple question on RNA-Seq terminology
      This question may be very simple and basic, but I just need to confirm that I understand the differences among those terminologies in the RNA-Seq context. Suppose I have a sample called SLR, and it is sequenced on 5 lanes, so I have (among other output files) BAM files like L1_SLR, L2_SLR, L3_SLR, L5_SLR and L7_SLR.bam. Here, the letter "L" denotes […]
    • FInding regions of interest with minimum coverage
      Hi, I have a bam file of all my accepted hits (tophat output) and an gtf file with my genes of interest for which I am trying to find potential antisense transcripts. I would like to create a list - preferably one that can be visualized in a genome browser - that shows all genes that have antisense reads in the accepted hits.bam file provided that there are […]
    • How to remove the intronic reads before counting
      I got RNASeq data in several samples. I checked the FastQC, seems the read quality are good (Hiseq 2000). But the problem is many reads are mapped to intronic region, and the regions have no any reference exons there (Refseq, ensembl, gencode). We don't know what they are. We guess the problem happend in library preparation, the concentration was low. N […]
    • Which strand of the mRNA molecule does the sequencer output as a "read"?
      In Illumina Stranded RNA-Seq (using the dUTP method), do the final reads in the fastq files correspond to the initial molecule (that was transcribed), or to the reverse complement of the molecule? C […]