The purpose of this tutorial is to guide users from the raw FASTQ files produced by the sequencer to obtaining aligned short reads to human and non-human (pathogen) references in the sequence Alignment/Map (SAM) report format. In this tutorial we will consider Illumina single end reads although most of this tutorial applies to pair end reads as well.
December 6, 2016
TACO – a computational method to reconstruct a consensus transcriptome from multiple RNA-seq data sets
November 23, 2016
Comprehensive evaluation of de novo transcriptome assembly programs and their effects on differential gene expression analysis
October 5, 2016
PseudoLasso – correctly align ambiguous reads and accurately estimate the expression level among homologous genes
July 22, 2016