The purpose of this tutorial is to guide users from the raw FASTQ files produced by the sequencer to obtaining aligned short reads to human and non-human (pathogen) references in the sequence Alignment/Map (SAM) report format. In this tutorial we will consider Illumina single end reads although most of this tutorial applies to pair end reads as well.
ASplice – a scalable and memory-efficient algorithm for de novo transcriptome assembly of non-model organisms
June 9, 2017
December 6, 2016
TACO – a computational method to reconstruct a consensus transcriptome from multiple RNA-seq data sets
November 23, 2016