Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. A team led by researchers at Stockholm University, Sweden have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. They demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.
- Ke R, Mignardi M, Pacureanu A, Svedlund J, Botling J, Wählby C, Nilsson M. (2013) In situ sequencing for RNA analysis in preserved tissue and cells. Nat Methods [Epub ahead of print]. [abstract]