EncodeThe ENCODE Consortium has finalized Standards, Guidelines and Best Practices for RNA-Seq V1.0

Download the document RNA-Seq Best Practices PDF

RNA-Seq is a directed experimental approach aimed at characterizing transcription in biological samples. This document presents a set of guidelines and standards focused on best practices for creating ‘reference quality’ transcriptome measurements. As technologies are rapidly evolving and the aims of RNA-Seq experiments are diverse, this document does not cover all standards and quality control issues.

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Comments

One Response to “Best Practices for RNA-Seq”

  1. Marco on July 13th, 2011 1:14 am

    30 M reads ??? PAIRED END ??? for differential espression analysis ? really is it the way you do it too ?
    Talking with Illumina’s tech support they suggest 10 M 50 bp single reads for human / mammal transcriptoms and 50-100 M 2X75 or 2X100 bp reads for transcript analysis (splicing sites variants and trascripts’ mutations’ analysis

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