- Date – 05 Jun 2013 – 07 Jun 2013
- Location – Wageningen, the Netherlands
- Keywords – RNA-seq, transcriptome, experimental design, statistics, data analysis, mapping, quantification, differential expression, interpretation, pathways
- Organiser – Experimental Plant Sciences (EPS, Wageningen UR) & NBIC
- Gabino Sanchez-Perez
- Edouard Severing
- Sandra Smit
- Ole Madsen
- Elio Schijlen
- Harm Nijveen
- Patrick Koks
- Sandra Smit
The Power of RNA-seq is an introductory course for researchers who want to use RNA-seq in their research project.
The course will focus on questions like:
- Which questions can be addressed with RNA-seq?
- How many samples and replicates do I need?
- Which steps are involved in an RNA-seq experiment?
- What is Differential Expression?
- What can go wrong?
This is a 3-day course that will consist of lectures in the morning and extensive hands-on computer practicals in the afternoon. You’ll learn about all aspects of RNA-seq during the morning lectures on NGS & RNA-seq theory, but also the context, applicability, power and expected results of RNA-seq experiments. During the practicals, you’ll learn the basic steps an RNA-seq pipeline consist of, how to interpret your data and to put the results to use in your research project. We’ll use Galaxy, R and webtools for this.
- experiental design
- sequencing requirements
- steps in RNA-seq data analysis (data quality control, transcript identification, quantification, differential expression, interpretation)
Researchers in Life Sciences (‘biologists’) starting with application of NextGen Sequencing & RNA-seq.
- No previous NGS experience is needed
- No command line or Linux computer experience is required
- No R-experience is required
For more information, contact Patrick Koks