ISRNA: an integrative online toolkit for short reads from high-throughput sequencing data.

Integrative Short Reads NAvigator (ISRNA) is an online toolkit for analyzing high-throughput small RNA sequencing data. Besides the high-speed genome mapping function, ISRNA provides statistics for genomic location, length distribution and nucleotide composition bias analysis of sequence reads. Number of reads mapped to known microRNAs and other classes of short non-coding RNAs, coverage of short reads on genes, expression abundance of sequence reads as well as some other analysis functions are also supported. The versatile search functions enable users to select sequence reads according to their sub-sequences, expression abundance, genomic location, relationship to genes, etc. A specialized genome browser is integrated to visualize the genomic distribution of short reads. ISRNA also supports management and comparison among multiple datasets.

rna-seq

AVAILABILITY: ISRNA is implemented in Java/C++/Perl/MySQL and can be freely accessed at http://omicslab.genetics.ac.cn/ISRNA/

CONTACT: xjwang@genetics.ac.cn

Luo GZ, Yang W, Ma YK, Wang XJ. (2013) ISRNA: an integrative online toolkit for short reads from high-throughput sequencing data. Bioinformatics [Epub ahead of print]. [abstract]