Amgen

Featured RNA-Seq Job – Scientist – Genome Analysis Unit – Amgen

Posted on May 14th, 2013

Auto req 18388BR Job Posting Title Scientist - Genome Analysis Unit Career Category Scientific Employee Subgroup Salaried…

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Clarient

Featured Job – Bioinformatician – GE (United States)

Posted on May 8th, 2013

Bioinformatician 1370806 CLARiENT, a GE Healthcare company At Clarient, we combine innovative diagnostic technologies with world-class pathology…

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Theranos

Theranos is currently looking for scientists with solid RNA Sequencing experience

Posted on May 8th, 2013

Please feel free to contact me at bhamory@theranos.com Theranos is actively building a world-class team. Ideal candidates are currently employed or…

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freiburg

Post-Doc Position in Systems Biology and Modeling of High-Throughput Data – Freiburg, Germany

Posted on Apr 22nd, 2013

The group “Systems Biology of the Cellular Microenvironment” at the Institute for Molecular Medicine and Cell Research (IMMZ), U, is…

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xylo

Featured RNA-Seq Job – Bioinformatics rna-seq proteomics Java developer

Posted on Apr 3rd, 2013

Job Description Xylo Technologies Inc. is an established Information Technology organization that serves Fortune and Mid-Tier corporations. Xylo has…

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infinity

RNA-Seq Summer Internship – Computational Biology

Posted on Mar 28th, 2013

Job Number: 83192857 Company Name: Infinity Pharmaceuticals, Inc. Location: Cambridge, MA US Career Focus: Management &…

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complextraitgenomics

PhD Position Available – Analyzing alternative splicing events in human transcriptome sequencing data

Posted on Mar 22nd, 2013

Supervisors Dr Qiongyi Zhao qiongyi.zhao@uq.edu.au Dr Kostya Shakhbazov k.shakhbazov@uq.edu.au A/Prof Naomi Wray…

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J&J

Featured RNA-Seq Job – Computational Biologist

Posted on Mar 21st, 2013

Position Title: SCIENTIST (Computational Biologist) Grade 26 Position ID: 1281130206 Description Johnson & Johnson Pharmaceutical…

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baylor

Featured RNA-Seq Job – Research Associate III Baylor Research Institute Dallas Bioinformatics\Computational Biology

Posted on Mar 6th, 2013

Research Associate III Baylor Research Institute Dallas Bioinformatics\Computational Biology Weekday Job Number: 81099147 Company…

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igmm

Featured Job – Postdoctoral Researcher (Molecular Genetics) Primordial Dwarfism and Organism Growth

Posted on Feb 26th, 2013

Postdoctoral Researcher (Molecular Genetics) Primordial Dwarfism and Organism Growth School of Molecular, Genetic and Population Health…

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pfizer

Senior / Principal Scientist / Data Analyst – Exploratory

Posted on Feb 22nd, 2013

Job Number: 79287699 Company Name: Pfizer Inc. Location: Cambridge, MA US Career Focus: Information Technology / Science &…

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jobs

Featured RNA-Seq Jobs

Posted on Feb 12th, 2013

Computational Biologist - Novartis Pharmaceuticals (United States) …in cell or molecular biology .Expertise in genomic data analysis -…

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life-tech

Internship Available – Product Development – RNA-Seq

Posted on Feb 8th, 2013

Intern Product Development Job Location: Austin, TX The successful candidate will support product development activities for gene expression…

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sck-cen

Master thesis – Bioinformatics – RNA-Seq analysis

Posted on Feb 6th, 2013

Belgian Nuclear Research Center Mol, Belgium Development of a RNA-seq analysis pipeline for bacterial transcriptomics Introduction/framework…

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morgridge

Featured RNA-Seq Job – Computational Biologist

Posted on Feb 5th, 2013

The Opportunity: The Morgridge Institute for Research seeks a Computational Biologist to provide bioinformatics support to researchers studying…

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pfizer

Featured Job – Senior Scientist – Pfizer

Posted on Jan 30th, 2013

Senior Scientist / Principal Scientist, Immunoregulation Job Number: 971088 Company Name: Pfizer Inc. Location: Cambridge, MA…

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ingenuity

Featured Job Listing – Senior Application Scientist – Ingenuity Systems

Posted on Jan 22nd, 2013

Senior Application Scientist Location: Redwood City, CA Job Code: 120146 # of…

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nuffield

Available Postdoctoral Position – RNA-Seq

Posted on Jan 8th, 2013

Postdoctoral Bioinformatician Nuffield Department of Medicine (NDM), Ludwig Institute for Cancer Research, Old Road Campus Research Building,…

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belcan

Featured Job Opening – Bioinformatics Scripter within Production Bioinformatics

Posted on Dec 17th, 2012

Job Number:                         165654 Category:                               A -…

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belfast

Available PhD Research Project – RNA-Seq

Posted on Nov 30th, 2012

Statistical analysis of RNA-seq data from next-generation sequencing experiments of cancer. Institution: Queen’s University…

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  • RSS SEQanswers – RNA Sequencing

    • The Transcript Length from Cufflinks May 25, 2013
      Hi Guys, I'm doing a fungus RNA-Seq.However, the merged transcriptome gave me very long transcripts (generally >2K). I used GeneMarES to do... […]
      hchang10
    • DESeq; can I omit timepoints during dispersal estimation? May 24, 2013
      I have a bacterial timecourse with 2 biological replicates per timepoint. There is a fair bit of variance between my replicates. I have spent the... […]
      amcloon
    • HT Seq Count stranded options May 24, 2013
      I am very new to bioinformatics, so I would be really grateful for some help! I have been using *HTSeq Count v0.5.3* and I am bit confused about... […]
      qwrissie
    • Tophat 2.0.8b installation error May 24, 2013
      I install tophat-2.0.8b to rerun the mapping. but when i make it, the error appears like this. make[1]: Entering directory... […]
      canhu
    • reason for low mapping rate?? May 23, 2013
      we did RNASeq using HiSeq 2000 100PE. When the data were back, I mapping them to the reference sequence, but got very low mapping rate (30-40%). I... […]
      miaom
    • cross-species data - questions about normalization May 23, 2013
      Hi, I have some data form various samples (cell types) in different species. I want to compare and analyze gene expression variability across the... […]
      trelek2
  • RSS Biostar – RNA-Seq

    • Why am I getting so many unmapped reads in STAR, classified as "too short"?
      I am currently using STAR to map several Hi-SEQ mRNA runs. I'm having trouble getting a decent amount of reads to map, but I don't really understand why. I'm hoping you can shed some light :) In the final log, only about 50% (or less) of the reads map to the reference. I'm using a GTF in addition to the genome. The unmapped bin that most […]
    • What are the best practices for SNP identification in RNA seq transcriptome data
      I have 20 RICE RNA seq tranascriptome data hiseq 2000 platform paired end reads. I aligned fasta reads with BWA and remove PCR duplicates with PICARD. Later I call SNP with samtools using various parameters. I would like to clarify what parameters should I used while alinging to reference rice genome for looking SNP location 100 bp upstream and 250 bp downst […]
    • How do TopHat options -g , --supress-hits, and Bowtie options interplay?
      Hi, I am currently using TopHat2 to map RNA-seq runs. I think there have been some changes pertaining the -g option. Does anyone know how it works now? I used to think that setting -g would look for n alignments for a given read, report them [if top-scoring] and discard those reads that had more than g [top scoring] alignments. Now, the description sounds mo […]
    • What happened to -k in TopHat for multiple-mapping reads?
      Selecting -g n in tophat does not discard reads mapping more than n, but instead only reports n alignments for those out all all their TOP scoring alignments. I think there used to be an option -k that would allow one to discard reads that topped x alignments -- whatever happened to that? I only see -g in the tophat 2 manual, no reporting options like before […]
    • Does tophat use the library-type information for mapping, or just for the XS flag?
      When I specify library-type to TopHat, i.e., first-strand, second-strand, unstranded, TopHat appends a value + or - to the XS:A flag, which is useful for subsequent analyses, such as annotation. However, does this information actually influence the "mappability" of reads, or is this unaffected? My thinking is that the information would be considere […]
    • Purpose of Y-shaped adapters in Illumina Sequencing?
      Hi all, Y adapters different sequences to be annealed to the 5' and 3' ends of each molecule in a library. The arms of the Y are unique, and the middle part, connected to the DNA fragment, is complementary. What are the advantages of this? My take of this over having fully-complementary adapters (ADAPTER1 - - - - - ADAPTER1) is that: -Upon primer a […]