Lexogen GmbH – Sample Preparation for RNA-Seq

Plant & Animal Genomes XXII – Industry Workshop

Sample preparation is an essential step in RNA sequencing experiments and any bias related to it may obscure the sequencing experiment results. The RNA extracted should be free from any contamination, including genomic DNA. Whenever possible the RIN should be higher than 8. For extraction of RNA of different sizes an appropriate method must be used. After the successful extraction a high quality strand-specific library preparation ensures that transcripts expressed from both strands of DNA are detected and distinguished in the RNA-Seq experiment. In our workshop we will discuss the major pitfalls in RNA-Seq sample prep and go into detail of the protocols of SPLIT RNA Extraction Kit and SENSE mRNA/RNA Library Prep Kits for your successful RNA-Seq experiment. We will also advise on how sample preparation can be evaluated with the existing quality control techniques.

Date: Monday, January 13, 2014
Time: 6:10 PM-8:20 PM
Room: Pacific Salon 1
Jekaterina Aleksejeva and Alexander Seitz
6:10 PM

Alexander Seitz, Lexogen GmbH

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