Maize is rich in genetic and phenotypic diversity. Understanding the sequence, structural, and expression variation that contributes to phenotypic diversity would facilitate more efficient varietal improvement. RNA based sequencing (RNA-seq) is a powerful approach for transcriptional analysis, assessing sequence variation, and identifying novel transcript sequences, particularly in large, complex, repetitive genomes such as maize. In this study, the authors sequenced RNA from whole seedlings of 21 maize inbred lines representing diverse North American and exotic germplasm.
- Hansey CN, Vaillancourt B, Sekhon RS, de Leon N, Kaeppler SM, Buell CR . (2012) Maize (Zea mays L.) Genome Diversity as Revealed by RNA-Sequencing. PLoS One 7(3), e33071. [article]