Next-generation sequencing technologies have had a dramatic impact in the field of genomic research through the provision of a low cost, high-throughput alternative to traditional capillary sequencers. These new sequencing methods have surpassed their original scope and now provide a range of utility-based applications, which allow for a more comprehensive analysis of the structure and content of microbial genomes than was previously possible. With the commercialization of a third generation of sequencing technologies imminent, the authors discuss the applications of current next-generation sequencing methods and explore their impact on and contribution to microbial genome research.
Next-generation sequencing technologies and their impact on microbial genomics
Forde BM, O’Toole PW. (2013) Next-generation sequencing technologies and their impact on microbial genomics. Brief Funct Genomics 12(5),440-53. [abstract]