RNA-Seq Blog

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Latest Poll Results

March 4, 2011 Leave a comment 1,662 Views

We asked: In the Year 2015, what will it cost to perform RNA-Seq (whole transcriptome sequencing – human sample)? You Answered: $10 17 13% $100 40 31% $500 38 29% ...

A Comparison of Single Molecule and Amplification Based Sequencing of Cancer Transcriptomes

March 3, 2011 Leave a comment 4,840 Views

The second wave of next generation sequencing technologies, referred to as single-molecule sequencing (SMS), carries the promise of profiling samples directly without employing polymerase chain reaction steps used by amplification-based ...

Range of NGS Applications Rises Quickly

March 3, 2011 Leave a comment 3,779 Views

By Vicki Glaser – Genetic Engineering News Advanced Technological Approach Generates Genomic Data Better, Faster, and Cheaper In the next-gen sequencing (NGS) arena, the focus over the past several years ...

The Tea Transcriptome

March 1, 2011 Leave a comment 3,713 Views

Tea is one of the most popular non-alcoholic beverages worldwide. However, the tea plant, Camellia sinensis, is difficult to culture in vitro, to transform, and has a large genome, rendering ...

SeqMap – A Tool For Mapping Millions Of Short Sequences To The Genome

March 1, 2011 Leave a comment 2,269 Views

SeqMap is a tool for identifying viral integration sites from LAM-PCR and LM-PCR analysis. The tool will extract vector sequence data then search existing genome databases for matches to the ...

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Featured RNA-SEQ Events

RNA-Seq Summit 2018

January 12, 2018

Single Cell RNA Sequencing – Workshop

December 22, 2017

London Calling 2018

October 22, 2017

RNA 2018 Conference

September 6, 2017

Jobs

Featured RNA-Seq Job – Director, Sequencing Research

17 days ago Leave a comment 578 Views

We seek a talented, collaborative inter-disciplinary scientist to lead innovative application of next-generation sequencing (NGS) methods in close collaboration with Celgene colleagues and ...
Read More »
Staff Bioinformatician (m/f) #CFBIO14

21 days ago Leave a comment 426 Views
Post-doc Position Available – Single Neuron Transcriptomics and Brain Regulation of Hunger

24 days ago Leave a comment 328 Views
Scientist I – Computational analysis of cell types in human brain using single nucleus RNA-sequencing

June 6, 2018 Leave a comment 500 Views
Scientists wanted – single cell RNA sequencing

May 31, 2018 Leave a comment 771 Views

Industry News

MNG Laboratories, Pronto Diagnostics partner to offer clinical RNA sequencing services in Israel

13 days ago Leave a comment 379 Views

MNG Laboratories, an Atlanta-based neurogenetic testing company, is pleased to announce that it has signed a Distribution Agreement with Pronto Diagnostics, a leading Israeli genomics company, to...
Read More »
Biogazelle appoints Armin Winands as new CEO

15 days ago Leave a comment 298 Views
Envisagenics wins SBIR grant for continued development of SpliceCore RNA-Seq analysis software

May 24, 2018 Leave a comment 553 Views
SomaGenics announced the launch of RealSeq®-biofluids

May 24, 2018 Leave a comment 425 Views
Dolomite Bio and Partek Collaborate on Single Cell Analysis Pipeline for Nadia Instrument

May 9, 2018 Leave a comment 891 Views

Biostars RNA-Seq
SEQanswers - RNA Sequencing

Rna-Seq Pipeline

So, there're papers on designing an RNA-seq experiment, and normalizing the data (Bullard et. a [...]

Visualisation Of Alternative Splicing Events Using Rna-Seq Data

Hi, I am looking for tools that provide a nice graphical display of different alternative splicing e [...]

How Are Rna-Seq Transcripts Assigned?

I am looking at some RNA-seq data analyzed by cufflinks using standard conditions, and trying to det [...]

Tophat Time And Memory Requirement

Hi, Anyone has an idea of memory and time requirement for tophat 1.4.1 for 76 paired-end reads (~60M [...]

Rna-Seq Data Variant Calling

Has anyone tried calling variants from RNA-seq data and comparing those with WGS/Exome sequencing va [...]

Tutorial: Workshop - Analysis Of Next Generation Sequencing Data

Workshop - Analysis of Next Generation Sequencing Data (Programming for Evolutionary Biology 2012 ht [...]

Searching For A Simple Yet Powerful Workflow For Rna-Seq

Hi, I have to start working with RNA-Seq data and have worked previously with ChIP-Seq so I am famil [...]

Rna-Seq Data For Cd34+ Cells Or An Alternative Approach To Compare My Expression Set

Hi! I am looking for a RNA-seq data on CD34+ cells (human), to compare the expression of genes in my [...]

Distribution Of Isoform Length

Is there any paper that contains the distribution of isoform length in different organisms? Here I [...]

Building A Webpage For Accessing Rna-Seq Expression Data

Hello all, I would like to get some hints to go about this task. I'd like to design a webpage/s [...]

Tophat Error : Oserror: [Errno 2] No Such File Or Directory

Hi, I am running tophat and getting a lot of different errors, I could understand some of them, but [...]

Tutorial: Visualizing Chip-Seq Data Using Ucsc [Bigwig]

Hola! I would like to write a quick tutorial on viewing your coverage/track/wiggle files using UCSC. [...]

Why Are There Many Rna-Seq Hits To Intronic Regions?

I am looking at RNA-seq data, which I have little experience in. I notice that for many genes, there [...]

Differential Rnaseq Expression Analysis Directly On Stanford'S Great?

Does anybody know if the tool GREAT from Stanford U. can be used for RNAseq differential expression [...]

De Novo Transcriptome And Comparative Genomics

Hello all, First time posting here. I am looking at doing some comparative genomics with a mammalian [...]

How To Get Equal Numbers Of Gene_Id And Exon_Id For Use In Newexoncountset Function For Dexseq

I'm trying to run DEXseq but am having trouble with the newExonCountSet function because my gen [...]

Is This Always The Case That The Wig/Bigwig/Bed Files Are Meant Only For Viewing Into Ucsc Browser?

Since I'm currently working with RNA-seq experiment data stored in wig/bigwig format. There are [...]

Strand-Specific Rna-Seq Data For Maqc Samples?

Hi, Is there publicly available strand-specific RNA-Seq data for the MAQC samples? It seems that the [...]

Publicly Available Strand-Specific Rna-Seq Data With Corresponding Rt-Pcr Data?

Hi, I'm wondering if there publicly available strand-specific RNA-Seq data with corresponding R [...]

Public Rnaseq/Dnaseq Data

Hi, I understand this might be a long shot, but : is anybody aware of a public dataset which contain [...]

Understanding sailfish-cir results

Hi, I have a problem trying to understand/interpret the results of the sailfish-cir. I went through [...]

TopHat v2.1.1- Warning: TopHat did not find any junctions in GTF file

I'm running TopHat v2.1.1 on Mycobacterium tuberculosis genome using the gtf file download from [...]

single-cell cDNA left 90 min at 95°C

Briefly, cDNA from single neurons prepared using SMART-Seq4 kit spent 90 minutes at 95°C due to huma [...]

EdgeR cannot fit a dispersion estimate trininty-RSEM-EdgeR

Hi everyone, I have some RNA-Seq data of 22 biological samples. I used RSEM after building a trinity [...]

Assembly vs mapping results

Hi guys, I performed RNA-seq analysis of bacterial transcriptome in four different stressed conditio [...]

What can you learn about your samples just by looking at normalized read counts?

Hi everyone, My study involves multiple cell lineages which were isolated from the mammary gland and [...]

human contamination for mouse RNAseq samples

I'm working on data analysis of mouse RNAseq samples now. The library was prepared by Nugen Uni [...]

Featured Presentations

RNA modifications poster – Discover the chemical structure of different RNA modifications and their distribution and function in mRNA and tRNAs

2 days ago

Evaluation of two main RNA-seq approaches for gene quantification in clinical RNA sequencing: polyA+ selection versus rRNA depletion

March 21, 2018