Post-doc position available – Single cell RNA-Seq of cell models

Salary £31,115 to £39,004pa plus excellent benefits.

Two year fixed-term position

A postdoctoral position is available at the Wellcome Trust Sanger Institute (WTSI) under the leadership of Dr Martin Hemberg (WTSI) and Dr Rick Livesey (Gurdon Institute). The position provides an exciting opportunity for the successful candidate to create multicellular stem cell-derived models of the human cerebral cortex. Using established directed differentiation protocols, cortical excitatory neurons, interneurons and microglia will be differentiated from induced pluripotent stem cells (iPSC) reprogrammed from patients harboring familial Alzheimer’s disease mutations and isogenic control lines. Single cell RNA sequencing of these models will be performed to provide insights into the neuronal and inflammatory pathways involved in Alzheimer’s Disease pathogenesis. The candidate will collaborate extensively during this process with the Teichmann and Liu groups (WTSI) in order to optimise the preparation of samples for single cell sequencing and interpret the data produced.

The post-holder will be expected to spend most of their time at the Gurdon Institute. The successful candidate will develop methods to create a complex co-culture system that incorporates these three major cell types to generate a more physiological model of Alzheimer’s disease initiation in vitro than those that are currently employed. We welcome candidates with a background in cell biology, molecular biology or developmental biology with extensive experience in performing cell and molecular biological assays. Experience in embryology or mammalian cell culture are highly desirable, as is a background with handling human iPSCs or immune cells.

The project will form part of an Open Targets (https://www.opentargets.org/) collaboration between the Sanger Institute, GlaxoSmithKline (GSK) and Biogen.

The successful candidate will have the opportunity to interact with experimental research and computational teams working at the Wellcome Trust/Cancer Research UK Gurdon Institute, the WTSI and at Biogen Inc. and will become part of team employing cutting edge cellular and transcriptomic techniques.

Essential Skills

  • Postgraduate qualification in Biological Sciences, or equivalent qualification
  • An excellent record of research publications in high impact journals
  • Extensive experience of core molecular and cell biology techniques
  • Extensive experience with and mammalian cell culture
  • Excellent organisational, communication skills and work ethic
  • Ability to independently develop, optimise and deploy research support, as a member of a closely collaborative team

Ideal Skills

  • A background in neurobiology and Alzheimer’s disease
  • Experience in handling human induced pluripotent stem cells
  • Experience in protocols for human IPS cell differentiation assays

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