Researchers at the USDA have generated and characterized a reference transcriptome for rainbow trout that represents multiple tissues responding to multiple stressors common to aquaculture production environments. This resource compliments existing public transcriptome data and will facilitate approaches aiming to evaluate gene expression associated with stress in this species.
Sequencing of a pooled normalized transcriptome library created from gill, brain, liver, spleen, kidney and muscle RNA of control and stressedfish produced 3,160,306 expressed sequence tags which were assembled and annotated. SNP discovery resulted in identification of ~58,000 putative single nucleotide polymorphisms including 24,479 which were predicted to fall within exons.
- C Sanchez CC, Weber GM, Gao G, Cleveland BM, Yao J, Rexroad CE. (2011) Generation of a reference transcriptome for evaluating rainbow trout responses to various stressors. BMC Genomics 12, 626. [article]