RNA-PET is a paired end tag (PET) sequencing method for full-length mRNA transcripts analysis using the next generation sequencer platforms such as Illumina GA and SOLiD. Unlike RNA-Seq method that sequences randomly sheared shotgun RNA short fragments, RNA-PET captures and sequences the 5′ and 3′ end tags of full-length cDNA fragments of all expressed genes in a biological sample. When mapped to reference genome, RNA-PET sequences can demarcate the boundaries of transcription units genome-wide, in addition to its ability to quantify the transcription level of each expression genes.
- Ruan X, Ruan Y. (2012) Genome Wide Full-Length Transcript Analysis Using 5′ and 3′ Paired-End-Tag Next Generation Sequencing (RNA-PET). Methods Mol Biol 809,535-62. [abstract]