Next generation sequencing is transforming our understanding of transcriptomes. It can determine the expression level of transcripts with a dynamic range of over six orders of magnitude from multiple tissues, developmental stages or conditions. Patterns of gene expression provide insight into functions of genes with unknown annotation.
This new RNA Seq-Atlas provides a record of high-resolution gene expression in a set of fourteen diverse tissues. The authors found dramatic tissue-specific gene expression of both the most highly-expressed genes and the genes specific to legumes in seed development and nodule tissues. Analysis of the gene expression profiles of over 2,000 genes with preferential gene expression in seed suggests there are more than 177 genes with functional roles that are involved in the economically important seed filling process.
The RNA-Seq atlas of Glycine max can be found here – http://www.soybase.org/soyseq
Severin AJ, Woody JL, Bolon YT, Joseph B, Diers BW, Farmer AD, Muehlbauer GJ, Nelson RT, Grant D, Specht JE, Graham MA, Cannon SB, May GD, Vance CP, Shoemaker RC. (2010) RNA-Seq Atlas of Glycine max: A guide to the soybean transcriptome. BMC Plant Biology 10, 160. [abstract]