Speaker: Lior Pachter, PhD
Location: Bren Hall 6011 UCI
Date: Friday, September 30, 2011 – 11:00 – 13:00
RNA-Seq is an encompassing term for methods that probe a transcriptome by high-throughput sequencing. We will provide an overview of RNA-Seq protocols, and will then focus on methods for analysis of RNA-Seq data. A recurring them will be the subtle, yet crucial connections between experimental design and analysis choices. We will focus on two tools we have developed: the Cufflinks suite for RNA-Seq reference assembly, quantification and differential expression, and eXpress which allows for rapid analysis of massive datasets. Results will be presented on a number of different datasets from human, mouse and Drosophila RNA-Seq experiments.