We’ve reached the magic number of 100 votes, so its time to retire the current poll and take a look at the results.
We asked – What sequencing depth is required for gene expression analysis? (i.e. Human sample, paired- end mappable reads, > 30 NT)
Results – Total Voters: 101
I think its interesting that the results do not fit to a bell curve. Also, as noted by a blog reader, the results could be biased due to the recent release of Best Practices for RNA-Seq by The ENCODE Consortium which recommends 30M reads for “experiments whose purpose is to evaluate the similarity between the transcriptional profiles of two polyA+ samples” and 100M – 200M for “experiments from a typical mammalian tissue or in which sensitivity of detection is important”.
Please check out our new poll in the right-hand sidebar and cast your vote.