RNA-seq techniques generate massive amounts of expression data. Several pipelines (e.g. Tophat and Cufflinks) are broadly applied to analyse these data sets. However, accessing and handling the analytical output remains challenging for non-experts.
Researchers at the Helmholtz Center Munich now present the RNASeqExpressionBrowser, an open-source web interface that can be used to access the output from RNA-seq expression analysis packages in different ways as it allows browsing for genes by identifiers, annotations or sequence similarity. Gene expression information can be loaded as long as it is represented in a matrix like format. Additionally, data can be made available by setting up the tool on a public server. For demonstration purposes, they have set up a version providing expression information from the barley genome.
AVAILABILITY: The source code and a show case are accessible at: http://mips.helmholtz-muenchen.de/plant/RNASeqExpressionBrowser/