Sequencing vs Microarrays for Transcriptome Analysis

From - Genomics Technologies: The Power of Genome-Scale Quantitative Data Resolution Profiling Transcriptomes, Plant Physiology 2010

…More recently, direct sequencing of transcripts by high-throughput sequencing technologies (RNA-Seq) has become an additional alternative to microarrays and is superseding SAGE and MPSS (Busch and Lohmann, 2007). Like SAGE and MPPS, RNA-Seq does not depend on genome annotation for prior probe selection and avoids biases introduced during hybridization of microarrays. On the other hand, RNA-Seq poses novel algorithmic and logistic challenges, and current wet-lab RNA-Seq strategies require lengthy library preparation procedures. Therefore, RNA-Seq is the method of choice in projects using nonmodel organisms and for transcript discovery and genome annotation. Because of their robust sample processing and analysis pipelines, often microarrays are still a preferable choice for projects that involve large numbers of samples for profiling transcripts in model organisms with well-annotated genomes.

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Table I. Advantages and disadvantages of various technologies for the measurement of transcript … abundance



Technologies


 


Advantages


 


Disadvantages


 


   
     
MPSS Sequences do not need to be known in advance Relatively expensive, laborious    
Microarrays Genome wide, relatively cheap, streamlined handling, oligos Sequences must be known in advance; limited sensitivity due to hybridization    
Quantitative reverse transcription-PCR High precision and high sensitivity Increasingly multiplexed Not genome wide; data normalization sensitive to method/choice of reference genes    
High-throughput sequencing Sequences do not need to be known in advance; possibility to sequence very short sequences Expensive at the moment, few solutions for downstream analysis; direct read out    

Baginsky S, Hennig L, Zimmermann P, Gruissem W. (2010) Gene expression analysis, proteomics, and network discovery. Plant Physiol 152(2) 402-10. [article]

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