Single-cell RNA-Seq Gaining Steam

Gene length and detection bias in single cell RNA sequencing protocols (5/24/2017) - Single cell RNA sequencing (scRNA-seq) has rapidly gained popularity for profiling transcriptomes of hundreds to thousands of single cells. This technology has led to the discovery of novel cell types and revealed insights into the development of complex tissues. However, many technical challenges need to be overcome during data generation. Due to minute amounts of ...
Computational approaches for interpreting scRNA-seq data (5/22/2017) - The recent developments in high throughput single-cell RNA sequencing technology (scRNA-seq) have enabled the generation of vast amounts of transcriptomic data at cellular resolution. With these advances come new modes of data analysis, building on high-dimensional data mining techniques. Here, researchers from the Wellcome Trust Sanger Institute and the European Bioinformatics Institute consider biological questions ...
rna-seq Probabilistic modeling of bifurcations in single-cell gene expression data using a Bayesian mixture of factor analyzers (5/19/2017) - Modeling bifurcations in single-cell transcriptomics data has become an increasingly popular field of research. Several methods have been proposed to infer bifurcation structure from such data, but all rely on heuristic non-probabilistic inference. Here University of Oxford researchers propose the first generative, fully probabilistic model for such inference based on a Bayesian hierarchical mixture of ...
rna-seq scRNA-Seq identifies of molecular markers for preoptic sleep neurons (5/18/2017) - In humans and other mammalian species, lesions in the preoptic area of the hypothalamus cause profound sleep impairment, indicating a crucial role of the preoptic area in sleep generation. However, the underlying circuit mechanism remains poorly understood. Electrophysiological recordings and c-Fos immunohistochemistry have shown the existence of sleep-active neurons in the preoptic area, especially in ...
rna-seq Sniffing out stem cell fates in the nose with single-cell RNA-Seq (5/16/2017) - Adult stem cells have the ability to transform into many types of cells, but tracing the path individual stem cells follow as they mature and identifying the molecules that trigger these fateful decisions are difficult in a living animal. UC Berkeley neuroscientists have now combined new techniques for sequencing the RNA in single cells with ...
rna-seq Analyzing single-cell RNA data in pre-malignant tumors (5/15/2017) - An interview with Dr Martin Hemberg, Wellcome Trust Sanger Institute, conducted by April Cashin-Garbutt, MA (Cantab) What is single-cell RNA sequencing and how can it help define cell types? RNA sequencing is basically the isolation of RNA from cells and the use of reverse transcriptase to turn the RNA into DNA. You can then use ...
rna-seq Single Cell RNA Sequencing Technologies – The Advantages of Being Single amongst Singles (5/12/2017) - Monday –  22 May 2017 12:00 pm – 1:00 pm Walter and Eliza Hall Institute of Medical Research – University of Melbourne In the past two decades high throughput DNA sequencing technologies have emerged and have rapidly become available to labs across the world. This technological breakthrough has given rise to many different applications that ...
JingleBells – A Repository of Immune-Related Single-Cell RNA-Sequencing Datasets (5/5/2017) - Recent advances in single-cell RNA-sequencing (scRNA-seq) technology increase the understanding of immune differentiation and activation processes, as well as the heterogeneity of immune cell types. Although the number of available immune-related scRNA-seq datasets increases rapidly, their large size and various formats render them hard for the wider immunology community to use, and read-level data are ...
rna-seq Topological RNA-Seq provides roadmap for cell development (5/2/2017) - Topological representation of four cellular populations during motor neuron differentiation. (Image credit: Rabadan lab/Columbia University Medical Cente Researchers at Columbia University Medical Center have created a new tool to describe the many possible ways in which a cell may develop. Rooted in the mathematical field of topology, the tool provides a roadmap that offers detailed ...
Single-cell RNA-seq reveals new types of human blood cells (4/24/2017) - Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, a team led by researchers at the Broad Institute of MIT and Harvard identified six ...

A slew of recent high profile papers and upcoming conferences highlight the growing interest in single-cell transcriptomics.