Single-cell RNA-Seq Gaining Steam

rna-seq University of Lausanne researchers identify a novel natural killer-like cell type with single-cell RNA-Seq (1/19/2017) - The immune system of vertebrate species consists of many different cell types that have distinct functional roles and are subject to different evolutionary pressures. Here, University of Lausanne and Sanger Institute researchers first analysed conservation of genes specific for all major immune cell types in human and mouse. Their results revealed higher gene turnover and ...
rna-seq MATQ-seq – effective detection of variation in single-cell transcriptomes (1/17/2017) - The development of single-cell RNA-seq has allowed the detection of gene expression at an anatomical resolution that is not accessible by bulk RNA-seq approaches. While single-cell RNA-seq methods have been successfully used to identify new cell types in complex tissues, technical noise in these methods is still substantial and affects researchers’ ability to detect subtle ...
rna-seq Analysing Single-Cell RNA-Seq data with SeqMonk (1/17/2017) - This video looks at how you can apply some of the tools within SeqMonk to the analysis of single-cell RNA-Seq data. It goes through QC, quantitation, variation analysis, clustering and PCA so show how these can all use useful in trying to understand the structure of large RNA-Seq datasets. – Babraham Bioinformatics
rna-seq A cost effective 5′ selective single cell transcriptome profiling approach with improved UMI design (1/16/2017) - Single cell RNA sequencing approaches are instrumental in studies of cell-to-cell variability. 5′ selective transcriptome profiling approaches allow simultaneous definition of the transcription start size and have advantages over 3′ selective approaches which just provide internal sequences close to the 3′ end. The only currently existing 5′ selective approach requires costly and labor intensive fragmentation ...
rna-seq New Technology for Single Cell RNA-Seq (1/12/2017) - Dolomite Bio has launched a new Injection Valve and Sample Loop for single cell RNA sequencing workflows. This option enables straightforward, gentle introduction of evenly distributed mRNA capture beads into the company’s RNA-Seq System for individual encapsulation of cells. The Injection Valve and Sample Loop is a complete module allowing barcoded mRNA capture beads in ...
rna-seq Illumina and Bio-Rad Launch Solution for Single-Cell Sequencing (1/10/2017) - Illumina, Inc. (NASDAQ:ILMN) and Bio-Rad Laboratories, Inc. (NYSE:BIO) (NYSE:BIOb) today announced the launch of the Illumina® Bio-Rad® Single-Cell Sequencing Solution at the J.P. Morgan Healthcare Conference. The comprehensive solution is the first next-generation sequencing (NGS) workflow for single-cell analysis, providing researchers the ability to investigate the coordinated contribution of individual cells in tissue function, disease ...
The Never-Ending Quest to Rewrite the Tree of Life (1/9/2017) - from Nova Next by Carrie Arnold – The bottom of the ocean is one of the most mysterious places on the planet, but microbiologist Karen Lloyd of the University of Tennessee, Knoxville, wanted to go deeper than that. In 2010, she was a postdoc at Aarhus University in Denmark, and Lloyd wanted to see what ...
rna-seq Post-doc Position Available – Single-Cell RNA-Seq (1/3/2017) - The Vogel lab invites applications for a postdoc position to study bacterial pathogenesis by several RNA-seq based approaches. The ultimate goal of this project is to achieve a high-resolution of gene expression on the single-cell level. For research themes and more information about methodology, see some of our recent publications: (Westermann AJ et al. (2016) ...
rna-seq switchde – Inference of switch-like differential expression along single-cell pseudotemporal trajectories (12/30/2016) - Pseudotime analyses of single-cell RNA-seq data have become increasingly common. Typically, a latent trajectory corresponding to a biological process of interest – such as differentiation or cell cycle – is discovered. However, relatively little attention has been paid to modelling the differential expression of genes along such trajectories. University of Oxford researchers present switchde, a ...
rna-seq Featured RNA-Seq Job – Bioinformatics Software Engineer – Single Cell Research Initiative (SCRI) (12/27/2016) - Job Description We are seeking an inventor with a creative and analytical mind to join Sloan Kettering Institute’s Single Cell Research Initiative (SCRI). The team’s goal is to develop cutting-edge algorithms to interpret the flood of data coming emerging single cell technologies to explore questions in tumor heterogeneity, metastasis and tumor immune environment. If you ...

A slew of recent high profile papers and upcoming conferences highlight the growing interest in single-cell transcriptomics.