Prof. J. Marioni, EMBL-EBI:
Studying cell-to-cell heterogeneity with single-cell RNA-seq
Dec 3, 12.10pm
Details: Single-cell RNA-sequencing (RNA-seq) promises to yield valuable insights about variability within populations of seemingly homogeneous cells. In this talk I will describe a quantitative statistical method to distinguish true biological variability from the high levels of technical noise in single-cell experiments. The approach allows quantification of the statistical significance of observed cell-to-cell variability in expression strength on a gene-by-gene basis. I will demonstrate the utility of the approach using data sets from A. thaliana and M. musculus before discussing new biological insights revealed by our analyses.