Tag Archives: alternative splicing

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High-Resolution Transcriptome Analysis with Long-Read RNA Sequencing

High-Resolution Transcriptome Analysis with Long-Read RNA Sequencing

RNA sequencing (RNA-seq) enables characterization and quantification of individual transcriptomes as well as detection of patterns of allelic expression and alternative splicing. Current RNA-seq protocols depend on high-throughput short-read sequencing of cDNA. However, as ongoing advances are rapidly yielding increasing ...

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51% of non-canonical splice sites are not annotated in GENCODE

51% of non-canonical splice sites are not annotated in GENCODE

Scientists at the Pontifical Catholic University of Chile have uncovered the diversity of non-canonical splice sites at the human transcriptome using deep transcriptome profiling. They mapped a total of 3.7 billion human RNA-seq reads and developed a set of stringent ...

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RNA-Seq a hot topic at last month’s ISMB conference

RNA-Seq a hot topic at last month’s ISMB conference

Poster – A50 Gene expression analyses of RNA-Sequencing data across multiple cancers to identify Basal-like cancer subtypes Kevin Thompson, Mayo Clinic, United States Xiaojia Tang, Mayo Clinic, United States Jason Sinwell, Mayo Clinic, United States Peter Vedell, Mayo Clinic, United ...

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