Tag Archives: alternative splicing

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Introduction to RNA-Seq Data Analysis

Introduction to RNA-Seq Data Analysis

  RNA-seq provide much more information than gene expression microarrays. In additional to gene expressions, it provides informa8on for: alternative splicing – structural changes of genes: Gene fusion. new genes/exons. splicing efficiency

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Kleat – cleavage site analysis of transcriptomes

Kleat – cleavage site analysis of transcriptomes

In eukaryotic cells, alternative cleavage of 3′ untranslated regions (UTRs) can affect transcript stability, transport and translation. For polyadenylated (poly(A)) transcripts, cleavage sites can be characterized with short-read sequencing using specialized library construction methods. However, for large-scale cohort studies as ...

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Strategies for transcriptional splice variant detection

Strategies for transcriptional splice variant detection

The advent and improvement of high-throughput sequencing over the past decade leveraged the study of whole genomes and transcriptomes of different organisms at lower costs. In transcriptomics, RNA-Seq expands our capacity to understand gene expression in different tissues and pathologies, ...

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High-Resolution Transcriptome Analysis with Long-Read RNA Sequencing

High-Resolution Transcriptome Analysis with Long-Read RNA Sequencing

RNA sequencing (RNA-seq) enables characterization and quantification of individual transcriptomes as well as detection of patterns of allelic expression and alternative splicing. Current RNA-seq protocols depend on high-throughput short-read sequencing of cDNA. However, as ongoing advances are rapidly yielding increasing ...

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