Tag Archives: alternative splicing

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RNA-Seq Blog – Poll Results

RNA-Seq Blog – Poll Results

We asked: What is your major application for RNA-Seq? Thanks to everyone who participated!  (64 respondents)  Check out the latest poll question in the sidebar.

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Insights from RNA Sequencing

Insights from RNA Sequencing

The human transcriptome comprises >80 000 protein-coding transcripts and the estimated number of proteins synthesized from these transcripts is in the range of 250 000 to 1 million. These transcripts and proteins are encoded by less than 20 000 genes, suggesting extensive regulation ...

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Introduction to RNA-Seq Data Analysis

Introduction to RNA-Seq Data Analysis

  RNA-seq provide much more information than gene expression microarrays. In additional to gene expressions, it provides informa8on for: alternative splicing – structural changes of genes: Gene fusion. new genes/exons. splicing efficiency

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Kleat – cleavage site analysis of transcriptomes

Kleat – cleavage site analysis of transcriptomes

In eukaryotic cells, alternative cleavage of 3′ untranslated regions (UTRs) can affect transcript stability, transport and translation. For polyadenylated (poly(A)) transcripts, cleavage sites can be characterized with short-read sequencing using specialized library construction methods. However, for large-scale cohort studies as ...

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