Tag Archives: cdna sequencing

TELP – a sensitive and versatile library construction method for next-generation sequencing

rna-seq

Next-generation sequencing has been widely used for the genome-wide profiling of histone modifications, transcription factor binding and gene expression through chromatin immunoprecipitated DNA sequencing (ChIP-seq) and cDNA sequencing (RNA-seq). Here, researchers from the Singapore Institute for Clinical Sciences describe a ...

Read More »

Step by Step – RNA-Seq

rna-seq

The introduction of RNA sequencing (RNA-seq), as a direct result of rapid progression of next-generation sequencing technologies, has revolutionized the world of transcriptomics. It enables quantification of the complete set of RNA with all its isoforms in a given cell, ...

Read More »

Allele-specific gene expression by RNA-seq

Variation in gene expression is thought to make a significant contribution to phenotypic diversity among individuals within populations. Although high-throughput cDNA sequencing offers a unique opportunity to delineate the genome-wide architecture of regulatory variation, new statistical methods need to be ...

Read More »