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RNA stability is an important determinant of eventual transcript levels

RNA stability is an important determinant of eventual transcript levels

Histone epigenome data determined by chromatin immunoprecipitation sequencing (ChIP-seq) is used in identifying transcript regions and estimating expression levels. However, this estimation does not always correlate with eventual RNA expression levels measured by RNA sequencing (RNA-seq). Part of the inconsistency ...

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ChIP-RNA-seq

ChIP-RNA-seq

Intro Biostatistics and Bioinformatics, lec 23 ChIP-seq and RNA-seq presented by Stuart Brown

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piPipes – a set of pipelines for piRNA and transposon analysis via small RNA-seq, RNA-seq, degradome- and CAGE-seq, ChIP-seq and genomic DNA sequencing

piPipes – a set of pipelines for piRNA and transposon analysis via small RNA-seq, RNA-seq, degradome- and CAGE-seq, ChIP-seq and genomic DNA sequencing

PIWI-interacting RNAs (piRNAs), 23-36 nt small silencing RNAs, repress transposon expression in the metazoan germ line, thereby protecting the genome. Although high-throughput sequencing has made it possible to examine the genome and transcriptome at unprecedented resolution, extracting useful information from ...

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RNA-Rocket: An RNA-Seq Analysis Resource for Infectious Disease Research

RNA-Rocket: An RNA-Seq Analysis Resource for Infectious Disease Research

RNA-Seq is a method for profiling transcription using high-throughput sequencing and is an important component of many research projects that wish to study transcript isoforms, condition specific expression, and transcriptional structure. The methods, tools, and technologies employed to perform RNA-Seq ...

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Next-generation sequencing turns 10!

Next-generation sequencing turns 10!

Ten years ago next-generation sequencing (NGS) technologies appeared on the market. During the past decade, tremendous progress has been made in terms of speed, read length, and throughput, along with a sharp reduction in per-base cost. Together, these advances democratized ...

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