Tag Archives: data analysis pipeline

omiRas – A MicroRNA-Seq Data Analysis Pipeline

rna-seq

To identify circulating miRNA biomarkers for AD, researchers from Meiji Pharmaceutical University, Japan reanalyzed a publicly available small RNA-Seq dataset, composed of blood samples derived from 48 AD patients and 22 normal control (NC) subjects, by a simple web-based miRNA ...

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Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

rna-seq

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient ...

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RNA-Seq with iReport and InSilico DB

rna-seq

RNA-Seq, or transcriptome sequencing, continues to be an exciting way to explore gene expression using next-generation sequencing (NGS). But for many researchers, the interpretation of RNA-Seq data remains a daunting task because they are using spreadsheets to read transcript names ...

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Study finds RNA-Seq, like microarrays, only accurate for relative expression, not absolute quantitation

rna-seq

The collborators at the SEQC/MAQC-III Consortium present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference ...

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Pipeline for Large RNA-Seq Projects

rna-seq

Steve Searle from the Sanger Institute talks about the pipeline for large RNA-seq projects. This workshop was filmed during a recent event, Computational Challenges and Performance Optimizations in NGS Data Analyses, jointly organized by Intel and the Francis Crick Institute. ...

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RNA-Seq Data Analysis – Where To Start?

In our last reader poll, we asked: Do we yet have a firm handle on the most appropriate/accurate pipeline for analysis of RNA-Seq datasets? The overwhelming result was: NO. N=72 We’re hearing feedback now from scientists developing data analysis methods ...

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