Tag Archives: de novo assembly

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Transcriptome Sequencing for the Detection of Chimeric Transcripts

Transcriptome Sequencing for the Detection of Chimeric Transcripts

The occurrence of chimeric transcripts has been reported in many cancer cells and seen as potential biomarkers and therapeutic targets. Modern high-throughput sequencing technologies offer a way to investigate individual chimeric transcripts and the systematic information of associated gene expressions ...

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Kleat – cleavage site analysis of transcriptomes

Kleat – cleavage site analysis of transcriptomes

In eukaryotic cells, alternative cleavage of 3′ untranslated regions (UTRs) can affect transcript stability, transport and translation. For polyadenylated (poly(A)) transcripts, cleavage sites can be characterized with short-read sequencing using specialized library construction methods. However, for large-scale cohort studies as ...

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De novo assembly of bacterial transcriptomes from RNA-seq data

De novo assembly of bacterial transcriptomes from RNA-seq data

A number of mature computational tools exist for both reference-based transcriptome assembly and de novo transcriptome assembly. However, most of the aforementioned tools were designed primarily for eukaryotic transcriptomes. Bacterial transcriptome assembly faces different challenges than eukaryotic transcriptome assembly. For ...

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Enabling large-scale next-generation sequence assembly with Blacklight

Enabling large-scale next-generation sequence assembly with Blacklight

A variety of extremely challenging biological sequence analyses were conducted on the XSEDE large shared memory resource Blacklight, using current bioinformatics tools and encompassing a wide range of scientific applications. These include genomic sequence assembly, very large metagenomic sequence assembly, ...

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SAT-Assembler – A Scalable and Accurate Targeted Gene Assembly Tool for Next-Generation Sequencing Data

SAT-Assembler – A Scalable and Accurate Targeted Gene Assembly Tool for Next-Generation Sequencing Data

Gene assembly, which recovers gene segments from short reads, is an important step in functional analysis of next-generation sequencing data. Lacking quality reference genomes, de novo assembly is commonly used for RNA-Seq data of non-model organisms and metagenomic data. However, ...

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