Tag Archives: deep sequencing

A detailed protocol for stranded RNA-sequencing

rna-seq

RNA-sequencing is a revolutionary tool to follow differential expression after treatment with cancer chemopreventive agents. It allows a real genome-wide screening independent of prior assumptions and is well suited for analyzing coding but also long noncoding RNAs. It still consents ...

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dRNA-Seq – Riboswitch Discovery by Combining RNA-Seq and Genome-Wide Identification of Transcriptional Start Sites

rna-seq

Deep-sequencing technologies applied to RNA have tremendous potential to identify novel transcripts with single-nucleotide resolution. By combining whole-transcript cDNA sequencing (RNA-seq) and genome-wide identification of transcription start sites (dRNA-seq), it is possible to characterize long 5′-untranslated regions potentially endowed with ...

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Transcription Start Site Associated RNAs (TSSaRNAs) Are Ubiquitous in All Domains of Life

rna-seq

A plethora of non-coding RNAs has been discovered using high-resolution transcriptomics tools, indicating that transcriptional and post-transcriptional regulation is much more complex than previously appreciated. Small RNAs associated with transcription start sites of annotated coding regions (TSSaRNAs) are pervasive in ...

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