Tag Archives: differential expression

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Past RNA-Seq Workshops – The Broad Institute

Past RNA-Seq Workshops – The Broad Institute

De novo & Genome-guided Transcript Reconstruction from RNA-Seq Participants in this workshop will learn the basics of how to reconstruct and analyze transcriptomes starting from RNA-Seq data using genome-guided and genome-free methods. Genome-guided reconstruction will be performed using the Tuxedo ...

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RNA-specific temporal dynamic methods still in infancy

RNA-specific temporal dynamic methods still in infancy

Interpreting gene expression profiles often involves statistical analysis of large numbers of differentially expressed genes, isoforms, and alternative splicing events at either static or dynamic spectrums. Reduced sequencing costs have made feasible dense time-series analysis of gene expression using RNA-seq; ...

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The blind spots of quantitative RNA-seq

The blind spots of quantitative RNA-seq

RNA-seq is now widely used to quantitatively assess gene expression, expression differences and isoform switching, and promises to deliver results for the entire transcriptome. However, whether the transcriptional state of a gene can be captured accurately depends critically on library ...

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