Tag Archives: gene expression

Gene Expression’s Big Rethink

rna-seq

Genetic Engineering News by Richard Stein – Two technologies used to interrogate gene expression, RNA-Seq and microarray analysis, often return strongly correlated results. These technologies, however, have not been evaluated for their concordance at the isoform level. To understand the ...

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Power analysis at the isoform level

rna-seq

RNA-Sequencing (RNA-Seq) has become a routine technology for investigating gene expression differences in comparative transcriptomic studies. Differential expression (DE) analysis of the isoforms of genes is just emerging now that expression (read counts) can be estimated with higher accuracy at ...

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Overview of methods and tools used to create and analyse co-expression networks constructed from gene expression data

Gene co-expression networks can be used to associate genes of unknown function with biological processes, to prioritize candidate disease genes or to discern transcriptional regulatory programmes. With recent advances in transcriptomics and next-generation sequencing, co-expression networks constructed from RNA sequencing ...

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RNA sequencing of archived dried blood spots

rna-seq

Neonatal dried blood spots (DBS) are routinely collected on standard Guthrie cards for all-comprising national newborn screening programs for inborn errors of metabolism, hypothyroidism and other diseases. In Denmark, the Guthrie cards are stored at – 20 °C in the ...

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