Tag Archives: gene expression

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Next-Generation Sequencing RNA-Seq Library Construction

Next-Generation Sequencing RNA-Seq Library Construction

This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA ...

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Cost comparison for qRT-PCR and RNA-Seq

Cost comparison for qRT-PCR and RNA-Seq

Gene expression studies are widely diffused in life science research. So far, the most popular techniques adopted for transcript quantification are Northern blot, reverse transcription polymerase chain reaction (RT-PCR), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), microarrays, and RNA ...

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FANTOM5 releases atlas of human gene expression

FANTOM5 releases atlas of human gene expression

FANTOM, a large international consortium led by RIKEN releases today the first comprehensive map of gene activity across the human body, and provides the first holistic view of the complex networks that regulate gene expression across the wide variety of ...

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RNA-specific temporal dynamic methods still in infancy

RNA-specific temporal dynamic methods still in infancy

Interpreting gene expression profiles often involves statistical analysis of large numbers of differentially expressed genes, isoforms, and alternative splicing events at either static or dynamic spectrums. Reduced sequencing costs have made feasible dense time-series analysis of gene expression using RNA-seq; ...

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A dynamic alternative splicing program regulates gene expression

A dynamic alternative splicing program regulates gene expression

Alternative pre-messenger RNA splicing remodels the human transcriptome in a spatiotemporal manner during normal development and differentiation. Here researchers from the University of California, Berkeley explored the landscape of transcript diversity in the erythroid lineage by RNA-seq analysis of five ...

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Reducing bias in RNA sequencing data: a novel approach to compute counts

Reducing bias in RNA sequencing data: a novel approach to compute counts

In the last decade, Next-Generation Sequencing technologies have been extensively applied to quantitative transcriptomics, making RNA sequencing a valuable alternative to microarrays for measuring and comparing gene transcription levels. Although several methods have been proposed to provide an unbiased estimate ...

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