Tag Archives: high-throughput sequencing

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Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient ...

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Scientists discover RNA modifications in some unexpected places

Scientists discover RNA modifications in some unexpected places

by Matt Fearer CAMBRIDGE, Mass. – The so-called central dogma of molecular biology—that DNA makes RNA which makes protein—has long provided a simplified explanation for how genetic information is deciphered and translated in living organisms. In reality, of course, the ...

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High-throughput sequencing for the study of bacterial pathogen biology

High-throughput sequencing for the study of bacterial pathogen biology

A revolution in sequencing technologies in recent years has led to dramatically increased throughput and reduced cost of bacterial genome sequencing. An increasing number of applications of the new technologies are providing broad insights into bacterial evolution, epidemiology, and pathogenesis. ...

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An efficient and sensitive method for preparing cDNA libraries from scarce biological samples

An efficient and sensitive method for preparing cDNA libraries from scarce biological samples

The preparation and high-throughput sequencing of cDNA libraries from samples of small RNA is a powerful tool to quantify known small RNAs (such as microRNAs) and to discover novel RNA species. Interest in identifying the small RNA repertoire present in ...

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