Tag Archives: high-throughput sequencing

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Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient ...

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Scientists discover RNA modifications in some unexpected places

Scientists discover RNA modifications in some unexpected places

by Matt Fearer CAMBRIDGE, Mass. – The so-called central dogma of molecular biology—that DNA makes RNA which makes protein—has long provided a simplified explanation for how genetic information is deciphered and translated in living organisms. In reality, of course, the ...

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