Tag Archives: isoforms

Gene expression should be analyzed on the isoform level

rna-seq

Alternative usage of transcript isoforms from the same gene has been hypothesized as an important feature in cancers. However, differential usage of gene transcripts between conditions – isoform switching – has not been comprehensively characterized in and across cancer types. ...

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Power analysis at the isoform level

rna-seq

RNA-Sequencing (RNA-Seq) has become a routine technology for investigating gene expression differences in comparative transcriptomic studies. Differential expression (DE) analysis of the isoforms of genes is just emerging now that expression (read counts) can be estimated with higher accuracy at ...

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The Pitfalls of RNA-Seq Methodology

rna-seq

TP53 undergoes multiple RNA-splicing events, resulting in at least nine mRNA transcripts encoding at least 12 functionally different protein isoforms. Antibodies specific to p53 protein isoforms have proven difficult to develop, thus researchers must rely on the transcript information to ...

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NMFP – a non-negative matrix factorization based preselection method to identify mRNA isoforms from RNA-seq data

rna-seq

The advent of next-generation RNA sequencing (RNA-seq) has greatly advanced transcriptomic studies, including system-wide identification and quantification of mRNA isoforms under various biological conditions. A number of computational methods have been developed to systematically identify mRNA isoforms in a high-throughput ...

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