Tag Archives: library preparation

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A protocol for sequencing the transcriptome of a single nuclei

A protocol for sequencing the transcriptome of a single nuclei

A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for ...

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Designing alternative splicing RNA-seq studies

Designing alternative splicing RNA-seq studies

esigning an RNA-seq study depends critically on its specific goals, technology and underlying biology, which renders general guidelines inadequate.  A team led by researchers at IRB Barcelona have developed a Bayesian framework to customize experiments so that goals can be ...

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