Tag Archives: library preparation

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Choosing a next-generation sequencing pipeline

Choosing a next-generation sequencing pipeline

The next-generation sequencing (NGS) technologies are revolutionary tools which have made possible achieving remarkable advances in genetics since the beginning of the twenty-first century. Thanks to the possibility to produce large amount of sequence data, these tools are going to ...

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Gene expression read results are influenced by RNA quality

Gene expression read results are influenced by RNA quality

Researchers at the University of Southern California examined the performance of three RNA-Sequencing library preparation protocols as a function of RNA integrity, comparing gene expressions between heat-degraded samples to their high-quality counterparts. This work is invaluable given the difficulty of ...

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Ovation RNA-Seq Targeted Depletion with InDA-C Technology

Ovation RNA-Seq Targeted Depletion with InDA-C Technology

<iframe width=”853″ height=”480″ src=”//www.youtube.com/embed/2Ph7V_lTFjg?rel=0″ frameborder=”0″ allowfullscreen></iframe> This video provides an overview of the InDA-C technology, used in NuGEN’s RNA-Seq workflows to enable depletion of unwanted transcripts from RNA-Seq libraries. Included are a description of the molecular mechanism and data from ...

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Detection of Plant Viruses in Natural Environments by Using RNA-Seq

Detection of Plant Viruses in Natural Environments by Using RNA-Seq

Sequencing of RNA by next generation sequencers, RNA-Seq, is revolutionizing virus detection. In addition to the unbiased detection of various viruses from wild plants in natural environments, RNA-Seq also allows for the parallel collection of host plant transcriptome data. Host ...

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Official release of TeloPrime Full-Length cDNA Amplification Kit

Official release of TeloPrime Full-Length cDNA Amplification Kit

TeloPrime enables the amplification of full-length cDNA with extreme stringency based on a new innovative technology. It allows more faithful tagging of full-length RNA transcripts that are both capped and polyadenylated compared to any other protocol available. The new technology ...

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