Tag Archives: library preparation

Feed Subscription

A protocol for sequencing the transcriptome of a single nuclei

A protocol for sequencing the transcriptome of a single nuclei

A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for ...

Read More »
Scroll To Top