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The blind spots of quantitative RNA-seq

The blind spots of quantitative RNA-seq

RNA-seq is now widely used to quantitatively assess gene expression, expression differences and isoform switching, and promises to deliver results for the entire transcriptome. However, whether the transcriptional state of a gene can be captured accurately depends critically on library ...

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RNA-sequencing from single nuclei

RNA-sequencing from single nuclei

It has recently been established that synthesis of double-stranded cDNA can be done from a single cell for use in DNA sequencing. Global gene expression can be quantified from the number of reads mapping to each gene, and mutations and ...

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